I have been until now downloading GEO data directly to my computer and using basic R functions to load tables and process them. It works, but I figured I would probably save time if I learn to use the GEOquery package, which looks promising. However, I'm failing tremendously at my first attempt. I can get a lot of good information, except the actual experiment data... and it seems to be there, but can't get to it! Example. I'm trying to get GSE19044, which contains 42 samples and uses the Illumina WG6 platform, which is great as I'm familiar with it. so I do: library(GEOquery) u = getGEO('GSE19044') show(u) > show(u) $GSE19044_series_matrix.txt.gz ExpressionSet (storageMode: lockedEnvironment) assayData: 45281 features, 42 samples element names: exprs protocolData: none phenoData sampleNames: GSM471318, GSM471319, ..., GSM471359 (42 total) varLabels and varMetadata description: title: NA geo_accession: NA ...: ... data_row_count: NA (39 total) featureData featureNames: ILMN_1212602, ILMN_1212603, ..., ILMN_3163582 (45281 total) fvarLabels and fvarMetadata description: ID: NA Species: NA ...: ... SPOT_ID: NA (31 total) additional fvarMetadata: Column, Description experimentData: use 'experimentData(object)' Annotation: GPL6887 It looks good. It looks like what I want is the 'assayData'. But I can't get to it. 'u' is a list, containing one element... > class(u) [1] "list" > length(u) [1] 1 > class(u[[1]]) [1] "ExpressionSet" attr(,"package") [1] "Biobase" ok, so I rename that, and look at its structure: eset<-u[[1]] str(eset) > str(eset) Formal class 'ExpressionSet' [package "Biobase"] with 7 slots ..@ assayData :<environment: 0x0645ec5c=""> ..@ phenoData :Formal class 'AnnotatedDataFrame' [package "Biobase"] [...] (omitted for brevity) I can extract the sample names, the basic annotation/probe identity etc easily: eset at phenoData@data #samples eset at featureData@data #annotation but how do I get into 'assayData'? from the 'show(u)' it looks like it contains what I am after: 45281 features, 42 samples ... but it's class 'environment' and that's throwing me off. I was looking into the GEOquery user guide, but I'm still none the wiser. How do I get in there? thanks for any help. Jose -- Dr. Jose I. de las Heras Email: J.delasHeras at ed.ac.uk The Wellcome Trust Centre for Cell Biology Phone: +44 (0)131 6507090 Institute for Cell & Molecular Biology Fax: +44 (0)131 6507360 Swann Building, Mayfield Road University of Edinburgh Edinburgh EH9 3JR UK -- The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336.

On Tue, Aug 16, 2011 at 7:20 AM, <j.delasheras at="" ed.ac.uk=""> wrote: > > I have been until now downloading GEO data directly to my computer and using > basic R functions to load tables and process them. > It works, but I figured I would probably save time if I learn to use the > GEOquery package, which looks promising. > > However, I'm failing tremendously at my first attempt. I can get a lot of > good information, except the actual experiment data... and it seems to be > there, but can't get to it! > > Example. I'm trying to get GSE19044, which contains 42 samples and uses the > Illumina WG6 platform, which is great as I'm familiar with it. > > so I do: > > library(GEOquery) > u = getGEO('GSE19044') > show(u) > >> show(u) > > $GSE19044_series_matrix.txt.gz > ExpressionSet (storageMode: lockedEnvironment) > assayData: 45281 features, 42 samples > ?element names: exprs > protocolData: none > phenoData > ?sampleNames: GSM471318, GSM471319, ..., GSM471359 ?(42 total) > ?varLabels and varMetadata description: > ? ?title: NA > ? ?geo_accession: NA > ? ?...: ... > ? ?data_row_count: NA > ? ?(39 total) > featureData > ?featureNames: ILMN_1212602, ILMN_1212603, ..., ILMN_3163582 ?(45281 total) > ?fvarLabels and fvarMetadata description: > ? ?ID: NA > ? ?Species: NA > ? ?...: ... > ? ?SPOT_ID: NA > ? ?(31 total) > ?additional fvarMetadata: Column, Description > experimentData: use 'experimentData(object)' > Annotation: GPL6887 > > It looks good. It looks like what I want is the 'assayData'. But I can't get > to it. > > 'u' is a list, containing one element... >> >> class(u) > > [1] "list" >> >> length(u) > > [1] 1 > >> class(u[[1]]) > > [1] "ExpressionSet" > attr(,"package") > [1] "Biobase" > > ok, so I rename that, and look at its structure: > > eset<-u[[1]] > str(eset) > >> str(eset) > > Formal class 'ExpressionSet' [package "Biobase"] with 7 slots > ?..@ assayData ? ? ? ?:<environment: 0x0645ec5c=""> > ?..@ phenoData ? ? ? ?:Formal class 'AnnotatedDataFrame' [package "Biobase"] > [...] (omitted for brevity) > > I can extract the sample names, the basic annotation/probe identity etc > easily: > eset at phenoData@data #samples > eset at featureData@data #annotation > > but how do I get into 'assayData'? > from the 'show(u)' it looks like it contains what I am after: 45281 > features, 42 samples ... but it's class 'environment' and that's throwing me > off. > > I was looking into the GEOquery user guide, but I'm still none the wiser. Hi, Jose. Sorry this was confusing for you. Your eset object above is an ExpressionSet and is one of the standard classes for storing gene expression data in Bioconductor; GEOquery uses this class where possible to store GEO data so as to facilitate downstream processing with other Bioconductor packages. Typically, you can get the expression data from an ExpressionSet by doing: assayDataElement(eset,'exprs') or the simpler shorthand: exprs(eset) Similarly, to get the sample variables, you can do: pData(eset) To get more help on ExpressionSet, you can do help("ExpressionSet-class") and read the related Biobase vignette. I hope that clears things up. Sean

you have noted that getGEO returns a list, and the first element of the list you got is an ExpressionSet instance. you are asking about access to the assayData. This concerns ExpressionSet mechanics, not GEOquery > library(Biobase) Welcome to Bioconductor Vignettes contain introductory material. To view, type 'browseVignettes()'. To cite Bioconductor, see 'citation("Biobase")' and for packages 'citation("pkgname")'. > data(sample.ExpressionSet) > assayData(sample.ExpressionSet) <environment: 0x100b1eb40=""> > ls(.Last.value) [1] "exprs" "se.exprs" > dim(assayData(sample.ExpressionSet)$exprs) [1] 500 26 > dim(assayData(sample.ExpressionSet)$se.exprs) [1] 500 26 > dim(exprs(sample.ExpressionSet)) [1] 500 26 assayData is not intended for direct handling by end users. the exprs() method will retrieve the matrix of expression values. On Tue, Aug 16, 2011 at 7:20 AM, <j.delasheras@ed.ac.uk> wrote: > > I have been until now downloading GEO data directly to my computer and > using basic R functions to load tables and process them. > It works, but I figured I would probably save time if I learn to use the > GEOquery package, which looks promising. > > However, I'm failing tremendously at my first attempt. I can get a lot of > good information, except the actual experiment data... and it seems to be > there, but can't get to it! > > Example. I'm trying to get GSE19044, which contains 42 samples and uses the > Illumina WG6 platform, which is great as I'm familiar with it. > > so I do: > > library(GEOquery) > u = getGEO('GSE19044') > show(u) > > show(u) >> > $GSE19044_series_matrix.txt.gz > ExpressionSet (storageMode: lockedEnvironment) > assayData: 45281 features, 42 samples > element names: exprs > protocolData: none > phenoData > sampleNames: GSM471318, GSM471319, ..., GSM471359 (42 total) > varLabels and varMetadata description: > title: NA > geo_accession: NA > ...: ... > data_row_count: NA > (39 total) > featureData > featureNames: ILMN_1212602, ILMN_1212603, ..., ILMN_3163582 (45281 total) > fvarLabels and fvarMetadata description: > ID: NA > Species: NA > ...: ... > SPOT_ID: NA > (31 total) > additional fvarMetadata: Column, Description > experimentData: use 'experimentData(object)' > Annotation: GPL6887 > > It looks good. It looks like what I want is the 'assayData'. But I can't > get to it. > > 'u' is a list, containing one element... > >> class(u) >> > [1] "list" > >> length(u) >> > [1] 1 > > class(u[[1]]) >> > [1] "ExpressionSet" > attr(,"package") > [1] "Biobase" > > ok, so I rename that, and look at its structure: > > eset<-u[[1]] > str(eset) > > str(eset) >> > Formal class 'ExpressionSet' [package "Biobase"] with 7 slots > ..@ assayData :<environment: 0x0645ec5c=""> > ..@ phenoData :Formal class 'AnnotatedDataFrame' [package > "Biobase"] [...] (omitted for brevity) > > I can extract the sample names, the basic annotation/probe identity etc > easily: > eset@phenoData@data #samples > eset@featureData@data #annotation > > but how do I get into 'assayData'? > from the 'show(u)' it looks like it contains what I am after: 45281 > features, 42 samples ... but it's class 'environment' and that's throwing me > off. > > I was looking into the GEOquery user guide, but I'm still none the wiser. > > How do I get in there? > > thanks for any help. > > Jose > > -- > Dr. Jose I. de las Heras Email: J.delasHeras@ed.ac.uk > The Wellcome Trust Centre for Cell Biology Phone: +44 (0)131 6507090 > Institute for Cell & Molecular Biology Fax: +44 (0)131 6507360 > Swann Building, Mayfield Road > University of Edinburgh > Edinburgh EH9 3JR > UK > > > -- > The University of Edinburgh is a charitable body, registered in > Scotland, with registration number SC005336. > > ______________________________**_________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/**listinfo/bioconductor<https: stat.et="" hz.ch="" mailman="" listinfo="" bioconductor=""> > Search the archives: http://news.gmane.org/gmane.** > science.biology.informatics.**conductor<http: news.gmane.org="" gmane.="" science.biology.informatics.conductor=""> > [[alternative HTML version deleted]]

Hi, Jose and Sean I try to run the exactly same code as you guys provided to extract GS19044 data. But I failed with connection issue. Here is my code and system information: R> source("http://www.bioconductor.org/biocLite.R") # It connected to the cite successfully BioC_mirror = http://bioconductor.org Change using chooseBioCmirror(). Warning messages: 1: In safeSource() : Redefining 'biocinstall' 2: In safeSource() : Redefining 'biocinstallPkgGroups' 3: In safeSource() : Redefining 'biocinstallRepos' library(Biobase) # no error message library(GEOquery) # no error message R> u = getGEO('GSE19044') Error in curlPerform(curl = curl, .opts = opts, .encoding = .encoding) : couldn't connect to host R> sessionInfo() R version 2.13.1 (2011-07-08) Platform: i386-pc-mingw32/i386 (32-bit) locale: [1] LC_COLLATE=English_United States.1252 [2] LC_CTYPE=English_United States.1252 [3] LC_MONETARY=English_United States.1252 [4] LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] tools stats graphics grDevices utils datasets methods [8] base other attached packages: [1] ff_2.2-3 bit_1.1-7 GEOquery_2.19.2 Biobase_2.12.2 loaded via a namespace (and not attached): [1] RCurl_1.6-7.1 XML_3.4-2.2 Daisy ________________________________ Electronic mail from Children's Mercy Hospitals and Clinics. This communication is intended only for the use of the addressee. It may contain information that is privileged or confidential under applicable law. If you are not the intended recipient or the agent of the recipient, you are hereby notified that any dissemination, copy or disclosure of this communication is strictly prohibited. If you have received this communication in error, please immediately forward the message to Children's Mercy Hospital's Information Security Officer via return electronic mail at informationsecurityofficer@cmh.edu and expunge this communication without making any copies. Thank you for your cooperation. [[alternative HTML version deleted]]