Probe ID to Sequence
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@khadeeja-ismail-4711
Last seen 8.8 years ago
Dear All, Could someone please tell me how I can get the sequence for any probe using the probe IDs in Illumina Human Methylation 450k data. Thanks in advance. Hajja [[alternative HTML version deleted]]
probe probe • 1.4k views
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@khadeeja-ismail-4711
Last seen 8.8 years ago
khadeeja ismail <hajjja at="" ...=""> writes: > > Dear All, > > Could someone please tell me how I can get the sequence for any probe using the probe IDs in Illumina Human > Methylation 450k data. > I have found one solution which is to replace the target IDs in the lumibatch object. Wonder if there are any other, such as any that would allow us to submit an array of target IDs and reurn the nuIDs. Regards, Hajja > > Dear All, > > Could someone please tell me how I can get the sequence for any probe using the probe IDs in Illumina Human > Methylation 450k data. > > Thanks in advance. > > Hajja > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at ... > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > >
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Forgot to cc: this to the list initially. Posting for completeness. Sequences are stored as NuIDs in the 450k package (at least for the time being...). > library(IlluminaHumanMethylation450k.db) > IlluminaHumanMethylation450kNUID[['cg00000292']] [1] "caXQ6wu1foImnrDw5U" > library(lumi) > id2seq( IlluminaHumanMethylation450kNUID[['cg00000292']] ) [1] "CGGCCTCAATGGTAAGTGTCCCTTGGAAGAGCGGCTGGTAATTAATGCCC" # first probe sequence in the manifest: > id2seq( IlluminaHumanMethylation450kNUID[['cg00035864']] ) == 'AGACACTAGCAGTCTTGTCCACATAGACCCTTGAATTTATCTCAAATTCG' [1] TRUE Granted, all this crap probably belongs in a separate .probe package... Thanks for the question Khadeeja and for the suggestion Herve. --t On Mon, Jul 25, 2011 at 2:56 AM, Khadeeja Ismail <hajjja@yahoo.com> wrote: > > khadeeja ismail <hajjja@...> writes: > > > > Dear All, > > > > Could someone please tell me how I can get the sequence for any probe > using > the probe IDs in Illumina Human > > Methylation 450k data. > > > > > I have found one solution which is to replace the target IDs in the > lumibatch > object. Wonder if there are any other, such as any that would allow us to > submit > an array of target IDs and reurn the nuIDs. > > Regards, > Hajja > > > > > > > Dear All, > > > > Could someone please tell me how I can get the sequence for any probe > using > the probe IDs in Illumina Human > > Methylation 450k data. > > > > Thanks in advance. > > > > Hajja > > > > [[alternative HTML version deleted]] > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@... > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > -- If people do not believe that mathematics is simple, it is only because they do not realize how complicated life is. John von Neumann<http: www-groups.dcs.st-="" and.ac.uk="" ~history="" biographies="" von_neumann.html=""> [[alternative HTML version deleted]]
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