Hello, I did normalisation on my .CEL files using the rma package. Now, do I need to have a separate file ( with probe/microRNA names and expression values (not log2 transformed)) ?? Is this is the file that I created in R called RData? What about the log2 transformed expression values? How do I check that the expression values are low?What do I do in that case? How do I check? Thank you, A.

Hi Ayse, Please read the posting guide: http://www.bioconductor.org/help/mailing-list/posting-guide/ and then re-post with the sort of information we will need to help you. Best, Jim On 7/7/2011 9:07 AM, Ayse Ulgen wrote: > Hello, > > I did normalisation on my .CEL files using the rma package. > > Now, do I need to have a separate file ( with probe/microRNA names > and expression values (not log2 transformed)) ?? Is this is the file > that I created in R called RData? > > What about the log2 transformed expression values? How do I check > that the expression values are low?What do I do in that case? How do > I check? > > Thank you, A. > > _______________________________________________ Bioconductor mailing > list Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor Search the > archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Douglas Lab University of Michigan Department of Human Genetics 5912 Buhl 1241 E. Catherine St. Ann Arbor MI 48109-5618 734-615-7826 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues