Hi All, I am new to R and trying to extract data from GEO database. It seems everything is fine with IMAC (desktop) when I practice the package GEOquery. However, there are problems when I try to use my PC labtop. I copy and paste one of my problems below: >gse2553 <- getGEO("GSE2553", GSEMatrix = TRUE) Found 1 file(s) GSE2553_series_matrix.txt.gz Error in file(con, "r") : cannot open the connection In addition: Warning messages: 1: In download.file(sprintf("ftp://ftp.ncbi.nih.gov/pub/geo/DATA/Serie sMatrix/%s/%s", : download had nonzero exit status 2: In file(con, "r") : cannot open file 'C:\DOCUME~1\huangji\LOCALS~1\Temp\RtmpmPEm8S/GSE25 53_series_matrix.txt.gz': No such file or directory > Can somebody help me ? Many thanks Jing [[alternative HTML version deleted]]