DESeq: Error: attempt to apply non-function
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João Moura ▴ 160
@joao-moura-4505
Last seen 10.2 years ago
Hi, Does anyone suspect why I'm getting this error? Thanks, > dim(c) [1] 7065 16 > group [1] "isowt" "isowt" "isowt" "isowt" "rlp" "rlp" "rlp" "rlp" "ssr" [10] "ssr" "ssr" "ssr" "xrlp" "xrlp" "xrlp" "xrlp" > cds <- newCountDataSet( c, group) > cds <- estimateSizeFactors( cds ) > cds <- estimateVarianceFunctions( cds ) Error: attempt to apply non-function > sessionInfo() R version 2.13.0 (2011-04-13) Platform: x86_64-unknown-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=C LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=en_US.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] tools stats graphics grDevices utils datasets methods [8] base other attached packages: [1] DESeq_1.4.0 locfit_1.5-6 lattice_0.19-23 [4] akima_0.5-4 org.Sc.sgd.db_2.5.0 org.EcK12.eg.db_2.5.0 [7] RSQLite_0.9-4 DBI_0.2-5 genefilter_1.34.0 [10] annotate_1.30.0 AnnotationDbi_1.14.1 rlecuyer_0.3-1 [13] snow_0.3-5 qpgraph_1.8.0 multicore_0.1-5 [16] Biobase_2.12.1 graph_1.30.0 igraph_0.5.5-2 loaded via a namespace (and not attached): [1] geneplotter_1.30.0 grid_2.13.0 Matrix_0.999375-50 RColorBrewer_1.0-2 [5] splines_2.13.0 survival_2.36-5 tcltk_2.13.0 xtable_1.5-6 -- João Moura [[alternative HTML version deleted]]
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@wolfgang-huber-3550
Last seen 12 weeks ago
EMBL European Molecular Biology Laborat…
Dear Jo?o can you (perhaps privately, or on a public filesharing place) make available to objects 'c' and 'group' so we can test this? Best wishes Wolfgang Jo?o Barbosa scripsit 05/18/2011 05:16 PM: > Hi, > > Does anyone suspect why I'm getting this error? > > Thanks, > > >> dim(c) > [1] 7065 16 >> group > [1] "isowt" "isowt" "isowt" "isowt" "rlp" "rlp" "rlp" "rlp" "ssr" > [10] "ssr" "ssr" "ssr" "xrlp" "xrlp" "xrlp" "xrlp" > >> cds<- newCountDataSet( c, group) >> cds<- estimateSizeFactors( cds ) >> cds<- estimateVarianceFunctions( cds ) > Error: attempt to apply non-function >> sessionInfo() > R version 2.13.0 (2011-04-13) > Platform: x86_64-unknown-linux-gnu (64-bit) > > locale: > [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C > [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 > [5] LC_MONETARY=C LC_MESSAGES=en_US.UTF-8 > [7] LC_PAPER=en_US.UTF-8 LC_NAME=C > [9] LC_ADDRESS=C LC_TELEPHONE=C > [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C > > attached base packages: > [1] tools stats graphics grDevices utils datasets methods > [8] base > > other attached packages: > [1] DESeq_1.4.0 locfit_1.5-6 lattice_0.19-23 > [4] akima_0.5-4 org.Sc.sgd.db_2.5.0 org.EcK12.eg.db_2.5.0 > [7] RSQLite_0.9-4 DBI_0.2-5 genefilter_1.34.0 > [10] annotate_1.30.0 AnnotationDbi_1.14.1 rlecuyer_0.3-1 > [13] snow_0.3-5 qpgraph_1.8.0 multicore_0.1-5 > [16] Biobase_2.12.1 graph_1.30.0 igraph_0.5.5-2 > > loaded via a namespace (and not attached): > [1] geneplotter_1.30.0 grid_2.13.0 Matrix_0.999375-50 > RColorBrewer_1.0-2 > [5] splines_2.13.0 survival_2.36-5 tcltk_2.13.0 xtable_1.5-6 > > > > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Wolfgang Huber EMBL http://www.embl.de/research/units/genome_biology/huber
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Hi Wolfgang, I dont know why but it is working now. I was doing some mistake that I cannot replicate again...sorry if it was an inconvenient false alarm. Best regards, On Thu, May 19, 2011 at 7:13 PM, Wolfgang Huber <whuber@embl.de> wrote: > Dear João > > can you (perhaps privately, or on a public filesharing place) make > available to objects 'c' and 'group' so we can test this? > > Best wishes > Wolfgang > > > João Barbosa scripsit 05/18/2011 05:16 PM: > >> Hi, >> >> Does anyone suspect why I'm getting this error? >> >> Thanks, >> >> >> dim(c) >>> >> [1] 7065 16 >> >>> group >>> >> [1] "isowt" "isowt" "isowt" "isowt" "rlp" "rlp" "rlp" "rlp" "ssr" >> [10] "ssr" "ssr" "ssr" "xrlp" "xrlp" "xrlp" "xrlp" >> >> cds<- newCountDataSet( c, group) >>> cds<- estimateSizeFactors( cds ) >>> cds<- estimateVarianceFunctions( cds ) >>> >> Error: attempt to apply non-function >> >>> sessionInfo() >>> >> R version 2.13.0 (2011-04-13) >> Platform: x86_64-unknown-linux-gnu (64-bit) >> >> locale: >> [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C >> [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 >> [5] LC_MONETARY=C LC_MESSAGES=en_US.UTF-8 >> [7] LC_PAPER=en_US.UTF-8 LC_NAME=C >> [9] LC_ADDRESS=C LC_TELEPHONE=C >> [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C >> >> attached base packages: >> [1] tools stats graphics grDevices utils datasets methods >> [8] base >> >> other attached packages: >> [1] DESeq_1.4.0 locfit_1.5-6 lattice_0.19-23 >> [4] akima_0.5-4 org.Sc.sgd.db_2.5.0 org.EcK12.eg.db_2.5.0 >> [7] RSQLite_0.9-4 DBI_0.2-5 genefilter_1.34.0 >> [10] annotate_1.30.0 AnnotationDbi_1.14.1 rlecuyer_0.3-1 >> [13] snow_0.3-5 qpgraph_1.8.0 multicore_0.1-5 >> [16] Biobase_2.12.1 graph_1.30.0 igraph_0.5.5-2 >> >> loaded via a namespace (and not attached): >> [1] geneplotter_1.30.0 grid_2.13.0 Matrix_0.999375-50 >> RColorBrewer_1.0-2 >> [5] splines_2.13.0 survival_2.36-5 tcltk_2.13.0 xtable_1.5-6 >> >> >> >> >> >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor@r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > > -- > > > Wolfgang Huber > EMBL > http://www.embl.de/research/units/genome_biology/huber > > _______________________________________________ > Bioconductor mailing list > Bioconductor@r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > -- João Moura [[alternative HTML version deleted]]
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