Hi Binita, Sorry to hear you've been having trouble installing limmaGUI and the Tcl/Tk extensions it needs. Generally the easiest way to install the extensions in Windows is to use ActiveTcl: http://www.activestate.com/Products/ActiveTcl/ ActiveTcl has a pretty good setup wizard. You should log in as administrator before running it so that it creates an entry in the Windows Registry. If you are trying to install limmaGUI without ActiveTcl, then you may be able to ignore the warning that ActiveTcl could not be found. I do have a bundled version of R + some Tcl/Tk stuff + limmaGUI, limma etc. somewhere on the limmaGUI site http://bioinf.wehi.edu.au/limmaGUI/ so if that's what you are trying to use, then maybe you can ignore the warning about ActiveTcl, but it certainly wouldn't hurt to install ActiveTcl. Always trying to improve installation methods, but there are various licensing and packaging issues I've had trouble with along the way. Hope this helps, James ---------------------------------------------------------------------- ---- James Wettenhall Tel: (+61 3) 9345 2629 Division of Genetics and Bioinformatics Fax: (+61 3) 9347 0852 The Walter & Eliza Hall Institute E-mail: wettenhall@wehi.edu.au of Medical Research, Mobile: (+61 / 0 ) 438 527 921 1G Royal Parade, Parkville, Vic 3050, Australia http://www.wehi.edu.au