Compatibility of Biorad cfx files to HTqPCR?
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TP Sahoo ▴ 10
@tp-sahoo-4577
Last seen 10.3 years ago
Hello everybody, I'm new to using R for qpcr data analyses. The machine I use is a BioRad cfx96, so I need some help with data import for HTqPCR. I understand .sds files from ABI is compatible for import. So,besides modifying the cfx to excel to txt file, what options do I have? I hope someone using a biorad qpcr may help me. Thanks, Tarini Sahoo -- Dept of Biological and Environmental Sciences University of Jyväskylä, Finland. FI-40500 [[alternative HTML version deleted]]
qPCR HTqPCR qPCR HTqPCR • 1.7k views
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Heidi Dvinge ★ 2.0k
@heidi-dvinge-2195
Last seen 10.3 years ago
Hi Tarini, I'm afraid that at the moment your only option is exporting your results to a tab delimited txt file. If there's a demand for it, it would be possible for me to add import functionality for cfx or other file formats to HTqPCR. I just haven't come across any of those files myself yet. Best \Heidi > Hello everybody, > > I'm new to using R for qpcr data analyses. The machine I use is a BioRad > cfx96, so I need some help with data import for HTqPCR. I understand .sds > files from ABI is compatible for import. > So,besides modifying the cfx to excel to txt file, what options do I have? > I hope someone using a biorad qpcr may help me. > > Thanks, > Tarini Sahoo > > -- > Dept of Biological and Environmental Sciences > University of Jyv?skyl?, Finland. FI-40500 > > > > > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor
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Hi Heidi, We recently got access to a BioRad CFX96 and CFX384 machine. I haven't used your HTqPCR package yet (we are still using a combination of BioRad's CFX software & Excel....) In other words, you can count me in for import functionality for these types of files. If needed, i can provide you with sample files from both the 96 and 384-well system. Regards, Guido --------------------------------------------------------- Guido Hooiveld, PhD Nutrition, Metabolism & Genomics Group Division of Human Nutrition Wageningen University Biotechnion, Bomenweg 2 NL-6703 HD Wageningen the Netherlands tel: (+)31 317 485788 fax: (+)31 317 483342 email: guido.hooiveld at wur.nl internet: http://nutrigene.4t.com http://www.researcherid.com/rid/F-4912-2010 -----Original Message----- From: bioconductor-bounces@r-project.org [mailto:bioconductor- bounces@r-project.org] On Behalf Of Heidi Dvinge Sent: Sunday, April 03, 2011 15:59 To: TP Sahoo Cc: bioconductor at r-project.org Subject: Re: [BioC] Compatibility of Biorad cfx files to HTqPCR? Hi Tarini, I'm afraid that at the moment your only option is exporting your results to a tab delimited txt file. If there's a demand for it, it would be possible for me to add import functionality for cfx or other file formats to HTqPCR. I just haven't come across any of those files myself yet. Best \Heidi > Hello everybody, > > I'm new to using R for qpcr data analyses. The machine I use is a > BioRad cfx96, so I need some help with data import for HTqPCR. I > understand .sds files from ABI is compatible for import. > So,besides modifying the cfx to excel to txt file, what options do I have? > I hope someone using a biorad qpcr may help me. > > Thanks, > Tarini Sahoo > > -- > Dept of Biological and Environmental Sciences > University of Jyv?skyl?, Finland. FI-40500 > > > > > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor _______________________________________________ Bioconductor mailing list Bioconductor at r-project.org https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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