Hi Iain, thanks for your interest in HTqPCR. In order to use plotCtArray you will indeed need version 1.5.2 or higher. It sounds like you have the version of HTqPCR back from when R-2.12.0 was the development version of R, i.e. ~1 year ago. The usual recommendation is to install packages using the instructions on http://www.bioconductor.org/install/ This makes sure you get the version compatible with your R-installation, and also install all dependencies. However, unless you run the current R-development version (R-2.13.0), this will install HTqPCR 1.4.0. Have you tried downloading the windows binaries from http://www.bioconductor.org/packages/devel/bioc/html/HTqPCR.html? I just borrowed a colleague's PC to test it, and if I download HTqPCR_1.5.3.zip and go to the R menu "Install packages" -> "Install package(s) from local zip files" it ran okay. Could you perhaps try the package from this webpage? If it doesn't work, then please copy-paste the exact error message, along with the output from sessionInfo(), so I can check the details of your R installation. HTH \Heidi > Dear Heidi, > > I'm trying to get HTqPCR up and running so we can try it out on our > Fluidigm data, it looks like it could be really useful! However, the > version I get from the Bioconductor install doesn't seem to have the > plotCtArray function, just looking at the "news" function I think it might > be version 1.3. I've tried downloading it directly from the EBI software > page for your group ( HTqPCR_1.5.2_Win32.zip) but when I try to install > from local zip it says "cannot open compressed file > 'HTqPCR_1.5.2_Win32/DESCRIPTION', probable reason 'No such file or > directory'".... I realise there is a good chance that this may be a fault > at my end (I'm still relatively new to R) but I was wondering if you had > any advice as to how I could go about correcting this, or at least easily > installing the latest version....as I say, the capacity to analyse > fluidigm data with this could be really useful for us, so do let me know > if you have a moment to spare to advise me on how to get this up and > running! If its something simple or obvious that I'm missing, maybe you > could point me in the right direction for some answers. > > Best wishes, > > Iain Macaulay > Weatherall Institute of Molecular Medicine, Oxford >