Dear friends, In my experiments there are 3 technical replications and 8 biology replications without dye swap. I have processed location and scale normalization for each print-tip-groups on 3 technical replications respectively. But next step what can I deal with them? Would I process scale normalization for 3 replications, and then take average or median of the 3 replications? Another question is how can I use these replications to find bad spots in 3 technical replications and 8 biology replication respectively. Maybe someone can give the whole idea of dealing with the replications. Thank a lots Zhao