Dear Mark, you got it! That was my problem, so simple but at the same time unthinkable for a biologist! Many thanks! That's why I love so much this list, you can solve pretty much any problem with Bioconductor by posting it here! Marcos On Thu, Dec 2, 2010 at 10:18 PM, Mark Cowley <m.cowley@garvan.org.au> wrote: > Hi Marcos, > i'm not familiar with oneChannelGUI, but I think the problem is that the > full path to that file has a space in it. Can you move your file to a > location with no spaces in the path & try again? ... or just rename > Microarray DATA to Microarray_DATA > > cheers, > Mark > > > On 03/12/2010, at 12:07 AM, Marcos Pinho wrote: > > Dear List, >> >> I am having some problems running oneChannelGUI to analyze some HuGene 1.0 >> ST arrays. >> >> I have installed all the necessary components according to the >> installation >> instructions. The program opens fine, I am able to select my working >> diretory and my RNA target file. The problem occurs when I try to >> normalize >> my data. I keep getting the following error message: >> >> >> Gene level probe sets summary started >> >> FATAL ERROR: .\TsvFile\TsvFile.cpp:2482: open: Could not open file: >> 'C:/Users/mbpinho/Documents/Microarray' to read. >> >> Gene level probe sets summary ended >> Warning messages: >> 1: In file(file, "rt") : only first element of 'description' argument used >> 2: In file(file, "rt") : >> cannot open file 'C:/Users/mbpinho/Documents/Microarray DATA/INCA >> Data/Uniscience/out6db54a37/': No such file or directory >> >>> >>> >> This problem only occurs when I am trying to analize the new Hugene 1.0 ST >> arrays, if instead I use other data with 3' IVT arrays, the software runs >> fine. I have already installed the APT tools from Affymetrix as well as >> the >> Tcl/Tk extensions. >> >> Any suggestions on how to fix my problem?? >> >> Regards, >> -- >> Marcos B. Pinho >> Programa de Engenharia Química - PEQ >> Laboratório de Engenharia de Cultivos Celulares- LECC >> Universidade Federal do Rio de Janeiro - UFRJ >> Instituto Nacional de Câncer - INCA >> Rio de Janeiro - Brasil >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor@r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > -- Marcos B. Pinho Programa de Engenharia Química - PEQ Laboratório de Engenharia de Cultivos Celulares- LECC Universidade Federal do Rio de Janeiro - UFRJ Instituto Nacional de Câncer - INCA Rio de Janeiro - Brasil [[alternative HTML version deleted]]