Normalization questions
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@anjan-purkayastha-4273
Last seen 10.2 years ago
>From reading the literature it seems that calculating the concordance correlation coefficient between two normalized arrays is a good measure of success of the normalization procedure. Is there a Bioconductor package that calculates this? Secondly, is there a bioconductor function that identifies outlier spot intensities among replicate arrays? Appreciate your response. Anjan -- =================================== anjan purkayastha, phd. research associate fas center for systems biology, harvard university 52 oxford street cambridge ma 02138 phone-703.740.6939 =================================== [[alternative HTML version deleted]]
Normalization Normalization • 1.2k views
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@sean-davis-490
Last seen 3 months ago
United States
On Thu, Sep 23, 2010 at 12:44 PM, ANJAN PURKAYASTHA < anjan.purkayastha@gmail.com> wrote: > >From reading the literature it seems that calculating the concordance > correlation coefficient between two normalized arrays is a good measure of > success of the normalization procedure. Is there a Bioconductor package > that > calculates this? > Hi, Anjan. The R cor() function can be used to calculate correlation. > Secondly, is there a bioconductor function that identifies outlier spot > intensities among replicate arrays? > This is a little vague. However, there are numerous packages for quality assessment of microarrays. You might try looking at some of those to see if they meet your needs for your experimental setup. Sean [[alternative HTML version deleted]]
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Correlation is not the same as "concordance correlation". Correlation measures where two data vectors follow some sort of straight line. Concordance measures whether they follow the identity line. On 9/23/2010 11:50 AM, Sean Davis wrote: > On Thu, Sep 23, 2010 at 12:44 PM, ANJAN PURKAYASTHA< > anjan.purkayastha at gmail.com> wrote: > >> > From reading the literature it seems that calculating the concordance >> correlation coefficient between two normalized arrays is a good measure of >> success of the normalization procedure. Is there a Bioconductor package >> that >> calculates this? >> > Hi, Anjan. The R cor() function can be used to calculate correlation. > > >> Secondly, is there a bioconductor function that identifies outlier spot >> intensities among replicate arrays? >> > This is a little vague. However, there are numerous packages for quality > assessment of microarrays. You might try looking at some of those to see if > they meet your needs for your experimental setup. > > Sean > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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On Fri, Sep 24, 2010 at 11:21 AM, Kevin R. Coombes < kevin.r.coombes@gmail.com> wrote: > Correlation is not the same as "concordance correlation". > > Good point. > > Correlation measures where two data vectors follow some sort of straight > line. > > Concordance measures whether they follow the identity line. > > > On 9/23/2010 11:50 AM, Sean Davis wrote: > >> On Thu, Sep 23, 2010 at 12:44 PM, ANJAN PURKAYASTHA< >> anjan.purkayastha@gmail.com> wrote: >> >> > From reading the literature it seems that calculating the concordance >>> correlation coefficient between two normalized arrays is a good measure >>> of >>> success of the normalization procedure. Is there a Bioconductor package >>> that >>> calculates this? >>> >>> Hi, Anjan. The R cor() function can be used to calculate correlation. >> >> >> Secondly, is there a bioconductor function that identifies outlier spot >>> intensities among replicate arrays? >>> >>> This is a little vague. However, there are numerous packages for >> quality >> assessment of microarrays. You might try looking at some of those to see >> if >> they meet your needs for your experimental setup. >> >> Sean >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor@stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
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@wolfgang-huber-3550
Last seen 3 months ago
EMBL European Molecular Biology Laborat…
Il Sep/23/10 6:44 PM, ANJAN PURKAYASTHA ha scritto: >> From reading the literature it seems that calculating the concordance > correlation coefficient between two normalized arrays is a good measure of > success of the normalization procedure. Is there a Bioconductor package that > calculates this? > Secondly, is there a bioconductor function that identifies outlier spot > intensities among replicate arrays? > Appreciate your response. > Anjan > Dear Anjan I'd be interested to hear what literature that is. If your statement were correct, then all normalisation methods should just be replaced by ACE (as has indeed been proposed, see Pubmed-ID 14633397, Faller et al., Normalization of DNA-microarray data by nonlinear correlation maximization.) Best wishes Wolfgang
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