Hello Heidi, Thank you very much for your quick reply. What you have listed is exactly what I was looking for. Your help is greatly appreciated. Best, Kee -----Original Message----- From: Heidi Dvinge [mailto:heidi@ebi.ac.uk] Sent: Friday, September 17, 2010 10:10 AM To: Kee Wong Cc: bioconductor at stat.math.ethz.ch Subject: Re: [BioC] HTqPCR plotCtRep only accepts 2 replicates Hello Kee, > Dear list members, > > Does anyone know if there are any functions like plotCtRep that can take > more than two technical replicates and look at the SD of the reps? > There currently isn't any function in HTqPCR for doing that - mainly since I've only worked with qPCR plates with max 2 replicates myself. However, I've been toying with the idea of adding something like a plot of mean (or median) versus standard deviation. Or perhaps just something like a boxplot of standard deviations within each plate. Would that be useful, or was it something else you had in mind? Cheers \Heidi > Best, > > Kee Wong > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor >

Thank you Heidi. You're the best! Kee -----Original Message----- From: Heidi Dvinge [mailto:heidi@ebi.ac.uk] Sent: Friday, September 17, 2010 10:32 AM To: Kee Wong Cc: bioconductor at stat.math.ethz.ch Subject: RE: [BioC] HTqPCR plotCtRep only accepts 2 replicates Ok. It'll probably take me a while to get some proper functions into the package, but I can send you some preliminary versions off-list within the next couple of days, so you can test them and see if they're suitable for you data/purpose. Cheers \Heidi > Hello Heidi, > > Thank you very much for your quick reply. > > What you have listed is exactly what I was looking for. Your help is > greatly appreciated. > > Best, > > Kee > > -----Original Message----- > From: Heidi Dvinge [mailto:heidi at ebi.ac.uk] > Sent: Friday, September 17, 2010 10:10 AM > To: Kee Wong > Cc: bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] HTqPCR plotCtRep only accepts 2 replicates > > Hello Kee, > >> Dear list members, >> >> Does anyone know if there are any functions like plotCtRep that can take >> more than two technical replicates and look at the SD of the reps? >> > There currently isn't any function in HTqPCR for doing that - mainly since > I've only worked with qPCR plates with max 2 replicates myself. > > However, I've been toying with the idea of adding something like a plot of > mean (or median) versus standard deviation. Or perhaps just something like > a boxplot of standard deviations within each plate. Would that be useful, > or was it something else you had in mind? > > Cheers > \Heidi > >> Best, >> >> Kee Wong >> >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > >