Dear List, I was using maSigPro for time series analysis of microarray. I have about 12 time points for a yeast data, and each time point only has two replicates. I am wondering if I can use quadratic model to perform the analysis. If I used higher order model, will overfitting become a problem? I noticed that in maSigPro tutorial, 4.4 mentioned "Long Time Series". But it has no detailed explaination. Does Ana or someone can share some examples I can follow for such analysis? Any other packages I can use for a long time series analysis? Your help will be greatly appreciated. Thanks again. Regards, Xiaokuan [[alternative HTML version deleted]]