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Peng Yu
▴
940
@peng-yu-3586
Last seen 10.3 years ago
Hi,
I see that I can use data() to load AffyBatch object from affydata, or
I could load cel files to get AffyBatch. But I'd rather construct an
example AffyBatch object using fake data so that I can understand how
AffyBatch works.
I adapted the example from Biobase (note that I don't load any data
from a file) which help me understand exactly the member functions and
the object construction. But I don't find such a tutorial for
AffyBatch. If it is available somewhere, would you please point it me.
If it is not, would give some examples so that I can understand how
AffyBatch is constructed and how the new member functions in AffyBatch
(as AffyBatch is inherent from ExpressionSet) is related to the
construction?
library('Biobase')
nFeatures=10
nSamples=4
exprs=matrix(runif(nFeatures*nSamples), nrow=nFeatures, ncol=nSamples)
colnames(exprs)=LETTERS[1:nSamples]
rownames(exprs)=letters[1:nFeatures]
set.seed(0)
varlabels=c('gender', 'type')
pData=data.frame(
sample(c('Female', 'Male'), nSamples, replace=T)
, sample(c('Control', 'Case'), nSamples, replace=T)
)
names(pData)=varlabels
row.names(pData)=LETTERS[1:nSamples]
metadata=data.frame(
labelDescription=c('Patient gender', 'Case/control status')
, row.names=varlabels
)
pData
metadata
phenoData=new('AnnotatedDataFrame', data=pData, varMetadata=metadata)
str(phenoData)
experimentData=new(
'MIAME',
name='Pierre Fermat',
lab='Francis Galton Lab',
contact='pfermat at lab.not.exist',
title='Smoking-Cancer Experiment',
abstract='An example ExpressionSet',
url='www.lab.not.exist',
other=list(notes='Created from text files')
)
annotation='hgu95av2'
eset=new('ExpressionSet', exprs=exprs, phenoData=phenoData,
experimentData=experimentData, annotation=annotation)
exprs(eset)
featureNames(eset)
sampleNames(eset)
phenoData(eset)
experimentData(eset)
--
Regards,
Peng
