Affymetrix HG U295 support in BioC?
2
0
Entering edit mode
@sole-acha-xavi-4144
Last seen 10.2 years ago
Dear listers, I would be pleased if someone could tell me if there are any plans to include the new Affymetrix HG U295 arrays in the list of supported platforms for "standard" affy analysis packages (affy, simpleaffy,...). Although the array came out a few months ago, it seems that the CDF has not been included in the BioC release yet. By now, the only BioC package I've found that seems to support these new array plates is xps. Does someone know anything more about this? Thank you very much for your help in advance. Best regards, ------ Xavier Sol? Acha Unitat de Biomarcadors i Susceptibilitat Unit of Biomarkers and Susceptibility Institut Catal? d'Oncologia // Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole (at) iconcologia.net
cdf affy xps cdf affy xps • 1.5k views
ADD COMMENT
0
Entering edit mode
@james-w-macdonald-5106
Last seen 42 minutes ago
United States
Hi Xavier, Sole Acha, Xavi wrote: > Dear listers, > > I would be pleased if someone could tell me if there are any plans to > include the new Affymetrix HG U295 arrays in the list of supported > platforms for "standard" affy analysis packages (affy, > simpleaffy,...). Although the array came out a few months ago, it > seems that the CDF has not been included in the BioC release yet. By > now, the only BioC package I've found that seems to support these new > array plates is xps. Does someone know anything more about this? I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219. Is that a typo on your part? If not, can you give a link to the correct webpage? I have built cdf and probe packages for the HG-U219 chip, but note that these will only work with the affy and affyPLM packages. Since the chips are PM-only, they won't work with simpleaffy, and will require some work on your part to get to work with gcrma, if at all. Best, Jim > > Thank you very much for your help in advance. > > Best regards, > > ------ Xavier Sol? Acha Unitat de Biomarcadors i Susceptibilitat Unit > of Biomarkers and Susceptibility Institut Catal? d'Oncologia // > Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 > L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 > / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole > (at) iconcologia.net > > _______________________________________________ Bioconductor mailing > list Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor Search the > archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Douglas Lab University of Michigan Department of Human Genetics 5912 Buhl 1241 E. Catherine St. Ann Arbor MI 48109-5618 734-615-7826 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues
ADD COMMENT
0
Entering edit mode
Hi Jim, Sorry... that was a mistake of mine. Certainly I was talking about U219 arrays. Are the cdf files you've built available? That would be of great help to me. Besides that... I guess there is a kind of mas5-like normalization for PM-only arrays... maybe newer versions of BioC may handle it. Thank you very much for your help. Xavi. -----Mensaje original----- De: James W. MacDonald [mailto:jmacdon at med.umich.edu] Enviado el: lunes, 28 de junio de 2010 18:18 Para: Sole Acha, Xavi CC: bioconductor at stat.math.ethz.ch Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? Hi Xavier, Sole Acha, Xavi wrote: > Dear listers, > > I would be pleased if someone could tell me if there are any plans to > include the new Affymetrix HG U295 arrays in the list of supported > platforms for "standard" affy analysis packages (affy, > simpleaffy,...). Although the array came out a few months ago, it > seems that the CDF has not been included in the BioC release yet. By > now, the only BioC package I've found that seems to support these new > array plates is xps. Does someone know anything more about this? I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219. Is that a typo on your part? If not, can you give a link to the correct webpage? I have built cdf and probe packages for the HG-U219 chip, but note that these will only work with the affy and affyPLM packages. Since the chips are PM-only, they won't work with simpleaffy, and will require some work on your part to get to work with gcrma, if at all. Best, Jim > > Thank you very much for your help in advance. > > Best regards, > > ------ Xavier Sol? Acha Unitat de Biomarcadors i Susceptibilitat Unit > of Biomarkers and Susceptibility Institut Catal? d'Oncologia // > Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 > L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 > / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole > (at) iconcologia.net > > _______________________________________________ Bioconductor mailing > list Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor Search the > archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Douglas Lab University of Michigan Department of Human Genetics 5912 Buhl 1241 E. Catherine St. Ann Arbor MI 48109-5618 734-615-7826 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues
ADD REPLY
0
Entering edit mode
Hi Xavi, What OS are you using? I can email the packages to you. Jim On 6/28/2010 1:19 PM, Sole Acha, Xavi wrote: > Hi Jim, > > Sorry... that was a mistake of mine. Certainly I was talking about U219 arrays. > > Are the cdf files you've built available? That would be of great help to me. > > Besides that... I guess there is a kind of mas5-like normalization for PM-only arrays... maybe newer versions of BioC may handle it. > > Thank you very much for your help. > > Xavi. > > > > -----Mensaje original----- > De: James W. MacDonald [mailto:jmacdon at med.umich.edu] > Enviado el: lunes, 28 de junio de 2010 18:18 > Para: Sole Acha, Xavi > CC: bioconductor at stat.math.ethz.ch > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? > > Hi Xavier, > > Sole Acha, Xavi wrote: >> Dear listers, >> >> I would be pleased if someone could tell me if there are any plans to >> include the new Affymetrix HG U295 arrays in the list of supported >> platforms for "standard" affy analysis packages (affy, >> simpleaffy,...). Although the array came out a few months ago, it >> seems that the CDF has not been included in the BioC release yet. By >> now, the only BioC package I've found that seems to support these new >> array plates is xps. Does someone know anything more about this? > > I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219. > Is that a typo on your part? > > If not, can you give a link to the correct webpage? > > I have built cdf and probe packages for the HG-U219 chip, but note that > these will only work with the affy and affyPLM packages. Since the chips > are PM-only, they won't work with simpleaffy, and will require some work > on your part to get to work with gcrma, if at all. > > Best, > > Jim > > >> >> Thank you very much for your help in advance. >> >> Best regards, >> >> ------ Xavier Sol? Acha Unitat de Biomarcadors i Susceptibilitat Unit >> of Biomarkers and Susceptibility Institut Catal? d'Oncologia // >> Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 >> L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 >> / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole >> (at) iconcologia.net >> >> _______________________________________________ Bioconductor mailing >> list Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the >> archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor > -- James W. MacDonald, M.S. Biostatistician Douglas Lab University of Michigan Department of Human Genetics 5912 Buhl 1241 E. Catherine St. Ann Arbor MI 48109-5618 734-615-7826 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues
ADD REPLY
0
Entering edit mode
Dear Xavier, Alternatively, you could use package xps, which also supports the HG-U219 arrays. It uses the official CDF and annotation files from Affymetrix to create so-called "root scheme files". However, you can download the pre-built scheme file "hgu219_na30.root" from: http://microarray.bu.edu/~sshen/xps/ Besides rma normalization xps also allows you to do mas5 normalization for PM-only arrays. Best regards Christian _._._._._._._._._._._._._._._._._._ C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a V.i.e.n.n.a A.u.s.t.r.i.a e.m.a.i.l: cstrato at aon.at _._._._._._._._._._._._._._._._._._ On 6/28/10 7:19 PM, Sole Acha, Xavi wrote: > Hi Jim, > > Sorry... that was a mistake of mine. Certainly I was talking about U219 arrays. > > Are the cdf files you've built available? That would be of great help to me. > > Besides that... I guess there is a kind of mas5-like normalization for PM-only arrays... maybe newer versions of BioC may handle it. > > Thank you very much for your help. > > Xavi. > > > > -----Mensaje original----- > De: James W. MacDonald [mailto:jmacdon at med.umich.edu] > Enviado el: lunes, 28 de junio de 2010 18:18 > Para: Sole Acha, Xavi > CC: bioconductor at stat.math.ethz.ch > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? > > Hi Xavier, > > Sole Acha, Xavi wrote: >> Dear listers, >> >> I would be pleased if someone could tell me if there are any plans to >> include the new Affymetrix HG U295 arrays in the list of supported >> platforms for "standard" affy analysis packages (affy, >> simpleaffy,...). Although the array came out a few months ago, it >> seems that the CDF has not been included in the BioC release yet. By >> now, the only BioC package I've found that seems to support these new >> array plates is xps. Does someone know anything more about this? > > I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219. > Is that a typo on your part? > > If not, can you give a link to the correct webpage? > > I have built cdf and probe packages for the HG-U219 chip, but note that > these will only work with the affy and affyPLM packages. Since the chips > are PM-only, they won't work with simpleaffy, and will require some work > on your part to get to work with gcrma, if at all. > > Best, > > Jim > > >> >> Thank you very much for your help in advance. >> >> Best regards, >> >> ------ Xavier Sol? Acha Unitat de Biomarcadors i Susceptibilitat Unit >> of Biomarkers and Susceptibility Institut Catal? d'Oncologia // >> Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 >> L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 >> / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole >> (at) iconcologia.net >> >> _______________________________________________ Bioconductor mailing >> list Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the >> archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >
ADD REPLY
0
Entering edit mode
@sole-acha-xavi-4144
Last seen 10.2 years ago
Dear Christian, thank you for your help. I will have a look at your package for sure. Actually, we already have it installed on our servers (along with root), and will try it with the 6 sample CEL files provided by Affy's website (I'm still waiting for our own data). Thank you so much! Xavi. -----Mensaje original----- De: cstrato [mailto:cstrato@aon.at] Enviado el: lun 28/06/2010 20:11 Para: Sole Acha, Xavi CC: James W. MacDonald; bioconductor@stat.math.ethz.ch Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? Dear Xavier, Alternatively, you could use package xps, which also supports the HG-U219 arrays. It uses the official CDF and annotation files from Affymetrix to create so-called "root scheme files". However, you can download the pre-built scheme file "hgu219_na30.root" from: / Besides rma normalization xps also allows you to do mas5 normalization for PM-only arrays. Best regards Christian _._._._._._._._._._._._._._._._._._ C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a V.i.e.n.n.a A.u.s.t.r.i.a e.m.a.i.l: cstrato at aon.at _._._._._._._._._._._._._._._._._._ On 6/28/10 7:19 PM, Sole Acha, Xavi wrote: > Hi Jim, > > Sorry... that was a mistake of mine. Certainly I was talking about U219 arrays. > > Are the cdf files you've built available? That would be of great help to me. > > Besides that... I guess there is a kind of mas5-like normalization for PM-only arrays... maybe newer versions of BioC may handle it. > > Thank you very much for your help. > > Xavi. > > > > -----Mensaje original----- > De: James W. MacDonald [mailto:jmacdon@med.umich.edu] > Enviado el: lunes, 28 de junio de 2010 18:18 > Para: Sole Acha, Xavi > CC: bioconductor@stat.math.ethz.ch > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? > > Hi Xavier, > > Sole Acha, Xavi wrote: >> Dear listers, >> >> I would be pleased if someone could tell me if there are any plans to >> include the new Affymetrix HG U295 arrays in the list of supported >> platforms for "standard" affy analysis packages (affy, >> simpleaffy,...). Although the array came out a few months ago, it >> seems that the CDF has not been included in the BioC release yet. By >> now, the only BioC package I've found that seems to support these new >> array plates is xps. Does someone know anything more about this? > > I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219. > Is that a typo on your part? > > If not, can you give a link to the correct webpage? > > I have built cdf and probe packages for the HG-U219 chip, but note that > these will only work with the affy and affyPLM packages. Since the chips > are PM-only, they won't work with simpleaffy, and will require some work > on your part to get to work with gcrma, if at all. > > Best, > > Jim > > >> >> Thank you very much for your help in advance. >> >> Best regards, >> >> ------ Xavier Solé Acha Unitat de Biomarcadors i Susceptibilitat Unit >> of Biomarkers and Susceptibility Institut Català d'Oncologia // >> Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 >> L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 >> / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole >> (at) iconcologia.net >> >> _______________________________________________ Bioconductor mailing >> list Bioconductor@stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the >> archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor > [[alternative HTML version deleted]]
ADD COMMENT
0
Entering edit mode
Dear Xavier, Actually, example 7 of script "script4xps.R", located in xps/examples, shows you how to import the 6 sample CEL files from Affymetrix. Best regards Christian On 6/28/10 9:05 PM, Sole Acha, Xavi wrote: > Dear Christian, > > thank you for your help. I will have a look at your package for sure. > Actually, we already have it installed on our servers (along with root), > and will try it with the 6 sample CEL files provided by Affy's website > (I'm still waiting for our own data). > > Thank you so much! > > Xavi. > > > -----Mensaje original----- > De: cstrato [mailto:cstrato at aon.at] > Enviado el: lun 28/06/2010 20:11 > Para: Sole Acha, Xavi > CC: James W. MacDonald; bioconductor at stat.math.ethz.ch > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? > > Dear Xavier, > > Alternatively, you could use package xps, which also supports the > HG-U219 arrays. It uses the official CDF and annotation files from > Affymetrix to create so-called "root scheme files". However, you can > download the pre-built scheme file "hgu219_na30.root" from: > / > > Besides rma normalization xps also allows you to do mas5 normalization > for PM-only arrays. > > Best regards > Christian > _._._._._._._._._._._._._._._._._._ > C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a > V.i.e.n.n.a A.u.s.t.r.i.a > e.m.a.i.l: cstrato at aon.at > _._._._._._._._._._._._._._._._._._ > > > On 6/28/10 7:19 PM, Sole Acha, Xavi wrote: > > Hi Jim, > > > > Sorry... that was a mistake of mine. Certainly I was talking about > U219 arrays. > > > > Are the cdf files you've built available? That would be of great help > to me. > > > > Besides that... I guess there is a kind of mas5-like normalization > for PM-only arrays... maybe newer versions of BioC may handle it. > > > > Thank you very much for your help. > > > > Xavi. > > > > > > > > -----Mensaje original----- > > De: James W. MacDonald [mailto:jmacdon at med.umich.edu] > > Enviado el: lunes, 28 de junio de 2010 18:18 > > Para: Sole Acha, Xavi > > CC: bioconductor at stat.math.ethz.ch > > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? > > > > Hi Xavier, > > > > Sole Acha, Xavi wrote: > >> Dear listers, > >> > >> I would be pleased if someone could tell me if there are any plans to > >> include the new Affymetrix HG U295 arrays in the list of supported > >> platforms for "standard" affy analysis packages (affy, > >> simpleaffy,...). Although the array came out a few months ago, it > >> seems that the CDF has not been included in the BioC release yet. By > >> now, the only BioC package I've found that seems to support these new > >> array plates is xps. Does someone know anything more about this? > > > > I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219. > > Is that a typo on your part? > > > > If not, can you give a link to the correct webpage? > > > > I have built cdf and probe packages for the HG-U219 chip, but note that > > these will only work with the affy and affyPLM packages. Since the chips > > are PM-only, they won't work with simpleaffy, and will require some work > > on your part to get to work with gcrma, if at all. > > > > Best, > > > > Jim > > > > > >> > >> Thank you very much for your help in advance. > >> > >> Best regards, > >> > >> ------ Xavier Sol? Acha Unitat de Biomarcadors i Susceptibilitat Unit > >> of Biomarkers and Susceptibility Institut Catal? d'Oncologia // > >> Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 > >> L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 > >> / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole > >> (at) iconcologia.net > >> > >> _______________________________________________ Bioconductor mailing > >> list Bioconductor at stat.math.ethz.ch > >> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the > >> archives: > >> http://news.gmane.org/gmane.science.biology.informatics.conductor > > > >
ADD REPLY
0
Entering edit mode
Dear Chris, I can't find such "example 7" in the file you mention, neither also in the other files in the "examples" folder. I'm using xps 1.4.10. Maybe it is located somewhere else? Thanks! Xavi. -----Mensaje original----- De: cstrato [mailto:cstrato at aon.at] Enviado el: lunes, 28 de junio de 2010 21:16 Para: Sole Acha, Xavi CC: James W. MacDonald; bioconductor at stat.math.ethz.ch; Cordero Romera, David Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? Dear Xavier, Actually, example 7 of script "script4xps.R", located in xps/examples, shows you how to import the 6 sample CEL files from Affymetrix. Best regards Christian On 6/28/10 9:05 PM, Sole Acha, Xavi wrote: > Dear Christian, > > thank you for your help. I will have a look at your package for sure. > Actually, we already have it installed on our servers (along with root), > and will try it with the 6 sample CEL files provided by Affy's website > (I'm still waiting for our own data). > > Thank you so much! > > Xavi. > > > -----Mensaje original----- > De: cstrato [mailto:cstrato at aon.at] > Enviado el: lun 28/06/2010 20:11 > Para: Sole Acha, Xavi > CC: James W. MacDonald; bioconductor at stat.math.ethz.ch > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? > > Dear Xavier, > > Alternatively, you could use package xps, which also supports the > HG-U219 arrays. It uses the official CDF and annotation files from > Affymetrix to create so-called "root scheme files". However, you can > download the pre-built scheme file "hgu219_na30.root" from: > / > > Besides rma normalization xps also allows you to do mas5 normalization > for PM-only arrays. > > Best regards > Christian > _._._._._._._._._._._._._._._._._._ > C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a > V.i.e.n.n.a A.u.s.t.r.i.a > e.m.a.i.l: cstrato at aon.at > _._._._._._._._._._._._._._._._._._ > > > On 6/28/10 7:19 PM, Sole Acha, Xavi wrote: > > Hi Jim, > > > > Sorry... that was a mistake of mine. Certainly I was talking about > U219 arrays. > > > > Are the cdf files you've built available? That would be of great help > to me. > > > > Besides that... I guess there is a kind of mas5-like normalization > for PM-only arrays... maybe newer versions of BioC may handle it. > > > > Thank you very much for your help. > > > > Xavi. > > > > > > > > -----Mensaje original----- > > De: James W. MacDonald [mailto:jmacdon at med.umich.edu] > > Enviado el: lunes, 28 de junio de 2010 18:18 > > Para: Sole Acha, Xavi > > CC: bioconductor at stat.math.ethz.ch > > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? > > > > Hi Xavier, > > > > Sole Acha, Xavi wrote: > >> Dear listers, > >> > >> I would be pleased if someone could tell me if there are any plans to > >> include the new Affymetrix HG U295 arrays in the list of supported > >> platforms for "standard" affy analysis packages (affy, > >> simpleaffy,...). Although the array came out a few months ago, it > >> seems that the CDF has not been included in the BioC release yet. By > >> now, the only BioC package I've found that seems to support these new > >> array plates is xps. Does someone know anything more about this? > > > > I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219. > > Is that a typo on your part? > > > > If not, can you give a link to the correct webpage? > > > > I have built cdf and probe packages for the HG-U219 chip, but note that > > these will only work with the affy and affyPLM packages. Since the chips > > are PM-only, they won't work with simpleaffy, and will require some work > > on your part to get to work with gcrma, if at all. > > > > Best, > > > > Jim > > > > > >> > >> Thank you very much for your help in advance. > >> > >> Best regards, > >> > >> ------ Xavier Sol? Acha Unitat de Biomarcadors i Susceptibilitat Unit > >> of Biomarkers and Susceptibility Institut Catal? d'Oncologia // > >> Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 > >> L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 > >> / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole > >> (at) iconcologia.net > >> > >> _______________________________________________ Bioconductor mailing > >> list Bioconductor at stat.math.ethz.ch > >> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the > >> archives: > >> http://news.gmane.org/gmane.science.biology.informatics.conductor > > > >
ADD REPLY
0
Entering edit mode
Dear Xavier, Your version of xps is much too old, I would suggest to upgrade to the current version xps_1.8.1. In any case you can find example 7 when you download the source of the newest version. Best regards Christian On 6/29/10 11:52 AM, Sole Acha, Xavi wrote: > Dear Chris, > > I can't find such "example 7" in the file you mention, neither also in the other files in the "examples" folder. I'm using xps 1.4.10. Maybe it is located somewhere else? > > Thanks! > > Xavi. > > > -----Mensaje original----- > De: cstrato [mailto:cstrato at aon.at] > Enviado el: lunes, 28 de junio de 2010 21:16 > Para: Sole Acha, Xavi > CC: James W. MacDonald; bioconductor at stat.math.ethz.ch; Cordero Romera, David > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? > > Dear Xavier, > > Actually, example 7 of script "script4xps.R", located in xps/examples, > shows you how to import the 6 sample CEL files from Affymetrix. > > Best regards > Christian > > > On 6/28/10 9:05 PM, Sole Acha, Xavi wrote: >> Dear Christian, >> >> thank you for your help. I will have a look at your package for sure. >> Actually, we already have it installed on our servers (along with root), >> and will try it with the 6 sample CEL files provided by Affy's website >> (I'm still waiting for our own data). >> >> Thank you so much! >> >> Xavi. >> >> >> -----Mensaje original----- >> De: cstrato [mailto:cstrato at aon.at] >> Enviado el: lun 28/06/2010 20:11 >> Para: Sole Acha, Xavi >> CC: James W. MacDonald; bioconductor at stat.math.ethz.ch >> Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? >> >> Dear Xavier, >> >> Alternatively, you could use package xps, which also supports the >> HG-U219 arrays. It uses the official CDF and annotation files from >> Affymetrix to create so-called "root scheme files". However, you can >> download the pre-built scheme file "hgu219_na30.root" from: >> / >> >> Besides rma normalization xps also allows you to do mas5 normalization >> for PM-only arrays. >> >> Best regards >> Christian >> _._._._._._._._._._._._._._._._._._ >> C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a >> V.i.e.n.n.a A.u.s.t.r.i.a >> e.m.a.i.l: cstrato at aon.at >> _._._._._._._._._._._._._._._._._._ >> >> >> On 6/28/10 7:19 PM, Sole Acha, Xavi wrote: >> > Hi Jim, >> > >> > Sorry... that was a mistake of mine. Certainly I was talking about >> U219 arrays. >> > >> > Are the cdf files you've built available? That would be of great help >> to me. >> > >> > Besides that... I guess there is a kind of mas5-like normalization >> for PM-only arrays... maybe newer versions of BioC may handle it. >> > >> > Thank you very much for your help. >> > >> > Xavi. >> > >> > >> > >> > -----Mensaje original----- >> > De: James W. MacDonald [mailto:jmacdon at med.umich.edu] >> > Enviado el: lunes, 28 de junio de 2010 18:18 >> > Para: Sole Acha, Xavi >> > CC: bioconductor at stat.math.ethz.ch >> > Asunto: Re: [BioC] Affymetrix HG U295 support in BioC? >> > >> > Hi Xavier, >> > >> > Sole Acha, Xavi wrote: >> >> Dear listers, >> >> >> >> I would be pleased if someone could tell me if there are any plans to >> >> include the new Affymetrix HG U295 arrays in the list of supported >> >> platforms for "standard" affy analysis packages (affy, >> >> simpleaffy,...). Although the array came out a few months ago, it >> >> seems that the CDF has not been included in the BioC release yet. By >> >> now, the only BioC package I've found that seems to support these new >> >> array plates is xps. Does someone know anything more about this? >> > >> > I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219. >> > Is that a typo on your part? >> > >> > If not, can you give a link to the correct webpage? >> > >> > I have built cdf and probe packages for the HG-U219 chip, but note that >> > these will only work with the affy and affyPLM packages. Since the chips >> > are PM-only, they won't work with simpleaffy, and will require some work >> > on your part to get to work with gcrma, if at all. >> > >> > Best, >> > >> > Jim >> > >> > >> >> >> >> Thank you very much for your help in advance. >> >> >> >> Best regards, >> >> >> >> ------ Xavier Sol? Acha Unitat de Biomarcadors i Susceptibilitat Unit >> >> of Biomarkers and Susceptibility Institut Catal? d'Oncologia // >> >> Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907 >> >> L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86 >> >> / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole >> >> (at) iconcologia.net >> >> >> >> _______________________________________________ Bioconductor mailing >> >> list Bioconductor at stat.math.ethz.ch >> >> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the >> >> archives: >> >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > >> >> >
ADD REPLY

Login before adding your answer.

Traffic: 708 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6