Dear Pedro and List. How straight and horizontal do the fits to mvaPlots does data normalized in AgiMicroRNA have to be to be reliable? Or to put it another way, how curved does the plot have to be before I should worry about a bad experiment and not trust the subsequent results. May I sent Pedro (or any other volunteer) extreme examples off-list. The ones I got look much worse than the ones in test set. Thanks and best wishes, Rich ------------------------------------------------------------ Richard A. Friedman, PhD Associate Research Scientist, Biomedical Informatics Shared Resource Herbert Irving Comprehensive Cancer Center (HICCC) Lecturer, Department of Biomedical Informatics (DBMI) Educational Coordinator, Center for Computational Biology and Bioinformatics (C2B2)/ National Center for Multiscale Analysis of Genomic Networks (MAGNet) Room 824 Irving Cancer Research Center Columbia University 1130 St. Nicholas Ave New York, NY 10032 (212)851-4765 (voice) friedman at cancercenter.columbia.edu http://cancercenter.columbia.edu/~friedman/ In Memoriam, George Scithers