Dear friends, I'm doing a project about microarray data normalization, and Arabidopsis thaliana gene is used as spiked control. But I'm very confused to use spiked controls, please give me some idea about it. Thanks a lot /Zhao

Spiked controls can be used to: 1) Determine the success of the hybridization. 2) Center arrays when there is reason to think that there may be a large percentage of genes that express differently on different arrays 3) Used in a titration series, the spiked controls can be used to at least somewhat calibrate the expression levels from the array with the actual RNA concentration. Regarding the last idea, I have not yet ploughed through all of the Bioconductor document - is there a routine to assist with this type of calibration? At 08:23 PM 12/15/2003, zhao luo wrote: >Dear friends, > >I'm doing a project about microarray data normalization, and Arabidopsis >thaliana gene is used as spiked control. But I'm very confused to use >spiked controls, please give me some idea about it. >Thanks a lot > >/Zhao > >_______________________________________________ >Bioconductor mailing list >Bioconductor@stat.math.ethz.ch >https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor Naomi S. Altman 814-865-3791 (voice) Associate Professor Bioinformatics Consulting Center Dept. of Statistics 814-863-7114 (fax) Penn State University 814-865-1348 (Statistics) University Park, PA 16802-2111