Hi All, I am trying to read raw intensity values from .gal, .gpr and .tiff files. I believe that these files are used for cDNA data. I would really appreciate if I could get some guidance or a starting point in doing this task using Bioconductor. Thanks, Tapan Mehta

Hi Tapan, Bioconductor doesn't offer any image analysis packages at the moment, so you will need to use Genepix or other sortware to convert your tiff files into raw intensity values. If you place you .gpr files and .gal files in the same directory, use the function gpTools to read them into R. library(marrayTools) rawdata <- gpTools(raw=TRUE) rawdata will be an object of marrayRaw. maM(rawdata) will give you the unnormalized log-ratios at this point. Jean On Mon, 15 Dec 2003, Tapan Mehta wrote: > Hi All, > > I am trying to read raw intensity values from .gal, > .gpr and .tiff files. I believe that these files are > used for cDNA data. I would really appreciate if I > could get some guidance or a starting point in doing > this task using Bioconductor. > > Thanks, > > Tapan Mehta > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >