Entering edit mode
Priyanka Jain
▴
20
@priyanka-jain-4005
Last seen 10.2 years ago
Hello,
I am using affy package of Biconductor.I have given raw cel
files
as input for normalization. I have to do background correction and
normalization of these files.
I am using the following commands:
> library(affy)
> Data<-ReadAffy()
> est<-rma(Data)
Background correcting
Normalizing
Calculating Expression
Warning message:
package 'hgu133acdf' was built under R version c(2, 0, 0) and help
will not
work correctly
Please re-install it
S4'
> write.exprs(est,file="backgroud_corrected")
Till here the raw cel file is being background corrected without any
error.
But when i am normalizing the data using quantiles method, using
following
command:
> nor<-normalize.AffyBatch.quantiles(Data)
The above command is running without any problem.But when i am trying
to
write the normalized file to a new file named"normalized"by the
command
given below, it is showing the following error.
> write.exprs(nor,file="normalized")
Error in function (classes, fdef, mtable) :
unable to find an inherited method for function "write.exprs", for
signature "AffyBatch"
Please try to solve this problem as early as possible.
With Regards,
Priyanka Jain
[[alternative HTML version deleted]]
