Hi, I am trying ti import into HTqPCR a TLDA with two sample, I have seen a previous post but I ma not able to make it works, it recogniseas a single sample but I have a two samples. I have isntalled HTqPCR 1.1.o on a windows machine # Plate Pos Flag Sample Detector Task Ct 1 Control A1 Passed Sample01 Gene1 Endogenous Control 11.463166 2 Control A2 Passed Sample01 Gene2 Target 33.949196 3 Control A3 Passed Sample01 Gene3 Target 27.956657 4 Control A4 Passed Sample02 Gene1 Target 26.92745 5 Control A5 Passed Sample02 Gene2 Target 24.992647 6 Control A6 Flagged Sample02 Gene3 Target 11.280299 An object of class "qPCRset" Size: 384 features, 1 samples Feature types: Endogenous Control, Target Feature names: CYP1A1-Hs00153120_m1 CYP1A1-Hs00153120_m1 CYP1A1-Hs00153120_m1 ... Feature classes: Feature categories: OK, Undetermined Sample names: Documents and Settings/fabbrmc/Desktop/card1/card1 NA NA ... Thank you Marco

Hello Marco, this feature is not available in HTqPCR 1.1.0, the release version. If you use a newer version, available either from the BioConductor development repository or http://www.ebi.ac.uk/bertone/software.html they'll be a parameter n.data in readCtData(). NB: Technically this is for the R devel version, but at least on my computer it works with R-2.10.0. HTH \Heidi On 16 Feb 2010, at 08:46, Marco Fabbri wrote: > Hi, > I am trying ti import into HTqPCR a TLDA with two sample, I have > seen a previous post but I ma not able to make it works, it > recogniseas a single sample but I have a two samples. > > I have isntalled HTqPCR 1.1.o on a windows machine > > > # Plate Pos Flag Sample Detector Task Ct > 1 Control A1 Passed Sample01 Gene1 Endogenous Control 11.463166 > 2 Control A2 Passed Sample01 Gene2 Target 33.949196 > 3 Control A3 Passed Sample01 Gene3 Target 27.956657 > 4 Control A4 Passed Sample02 Gene1 Target 26.92745 > 5 Control A5 Passed Sample02 Gene2 Target 24.992647 > 6 Control A6 Flagged Sample02 Gene3 Target 11.280299 > > An object of class "qPCRset" > Size: 384 features, 1 samples > Feature types: Endogenous Control, Target > Feature names: CYP1A1-Hs00153120_m1 CYP1A1-Hs00153120_m1 > CYP1A1-Hs00153120_m1 ... > Feature classes: > Feature categories: OK, Undetermined > Sample names: Documents and > Settings/fabbrmc/Desktop/card1/card1 NA NA ... > > > Thank you > > Marco > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/ > gmane.science.biology.informatics.conductor