Problem with MAS5 calls on MoEx array
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@peter-saffrey-3645
Last seen 10.3 years ago
I am using the affy library with mouseexonpmcdf to compute RMA and MAS5 calls. The RMA works fine. The MAS5 throws errors: > library("affy") Loading required package: Biobase Welcome to Bioconductor Vignettes contain introductory material. To view, type 'openVignette()'. To cite Bioconductor, see 'citation("Biobase")' and for packages 'citation(pkgname)'. > adata <- ReadAffy() > adata at cdfName <- "mouseexonpmcdf" > pmaCalls <- mas5(adata) background correction: mas PM/MM correction : mas expression values: mas background correcting...Error in as.vector(data) : NA/NaN/Inf in foreign function call (arg 1) > sessionInfo() R version 2.9.2 (2009-08-24) x86_64-pc-linux-gnu locale: C attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] mouseexonpmcdf_1.1 affy_1.22.1 Biobase_2.4.1 loaded via a namespace (and not attached): [1] affyio_1.12.0 preprocessCore_1.6.0 tools_2.9.2 My CEL file can be found here: http://cs-analysis.clinmed.gla.ac.uk/MoEx/KC4MoEX1E143.CEL Peter
cdf affy cdf affy • 1.6k views
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cstrato ★ 3.9k
@cstrato-908
Last seen 6.2 years ago
Austria
Dear Peter, Please note that exon arrays have only PM probesets and no MM probesets, thus usually it is not possible to do mas5 with these arrays. As far as I know my BioC package "xps" is the only package where you can indeed apply mas5 to exon arrays. Best regards Christian _._._._._._._._._._._._._._._._._._ C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a V.i.e.n.n.a A.u.s.t.r.i.a e.m.a.i.l: cstrato at aon.at _._._._._._._._._._._._._._._._._._ Peter Saffrey wrote: > I am using the affy library with mouseexonpmcdf to compute RMA and MAS5 calls. > The RMA works fine. The MAS5 throws errors: > > >> library("affy") >> > Loading required package: Biobase > > Welcome to Bioconductor > > Vignettes contain introductory material. To view, type > 'openVignette()'. To cite Bioconductor, see > 'citation("Biobase")' and for packages 'citation(pkgname)'. > > >> adata <- ReadAffy() >> adata at cdfName <- "mouseexonpmcdf" >> pmaCalls <- mas5(adata) >> > background correction: mas > PM/MM correction : mas > expression values: mas > background correcting...Error in as.vector(data) : NA/NaN/Inf in foreign > function call (arg 1) > >> sessionInfo() >> > R version 2.9.2 (2009-08-24) > x86_64-pc-linux-gnu > > locale: > C > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] mouseexonpmcdf_1.1 affy_1.22.1 Biobase_2.4.1 > > loaded via a namespace (and not attached): > [1] affyio_1.12.0 preprocessCore_1.6.0 tools_2.9.2 > > > My CEL file can be found here: > > http://cs-analysis.clinmed.gla.ac.uk/MoEx/KC4MoEX1E143.CEL > > Peter > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > >
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Hi, Thanks for the recommendation. This seems to bring its own problems. Here is the error from trying to install xps. g++ -O2 -Wall -fPIC -pthread -m64 -I/usr/include/root -c XPSUtils.cxx XPSUtils.cxx: In function 'void READ_WSTRING(std::ifstream&, char*&, Bool_t)': XPSUtils.cxx:4611: error: 'wcstombs' was not declared in this scope make: *** [XPSUtils.o] Error 1 ERROR: compilation failed for package 'xps' $ uname -a Linux cs-analysis 2.6.31-17-generic #54-Ubuntu SMP Thu Dec 10 17:01:44 UTC 2009 x86_64 GNU/Linux $ gcc --version gcc (Ubuntu 4.4.1-4ubuntu9) 4.4.1 Cheers, Peter ________________________________________ From: cstrato [cstrato@aon.at] Sent: 08 February 2010 21:44 To: Peter Saffrey Cc: bioconductor at stat.math.ethz.ch Subject: Re: [BioC] Problem with MAS5 calls on MoEx array Dear Peter, Please note that exon arrays have only PM probesets and no MM probesets, thus usually it is not possible to do mas5 with these arrays. As far as I know my BioC package "xps" is the only package where you can indeed apply mas5 to exon arrays. Best regards Christian _._._._._._._._._._._._._._._._._._ C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a V.i.e.n.n.a A.u.s.t.r.i.a e.m.a.i.l: cstrato at aon.at _._._._._._._._._._._._._._._._._._ Peter Saffrey wrote: > I am using the affy library with mouseexonpmcdf to compute RMA and MAS5 calls. > The RMA works fine. The MAS5 throws errors: > > >> library("affy") >> > Loading required package: Biobase > > Welcome to Bioconductor > > Vignettes contain introductory material. To view, type > 'openVignette()'. To cite Bioconductor, see > 'citation("Biobase")' and for packages 'citation(pkgname)'. > > >> adata <- ReadAffy() >> adata at cdfName <- "mouseexonpmcdf" >> pmaCalls <- mas5(adata) >> > background correction: mas > PM/MM correction : mas > expression values: mas > background correcting...Error in as.vector(data) : NA/NaN/Inf in foreign > function call (arg 1) > >> sessionInfo() >> > R version 2.9.2 (2009-08-24) > x86_64-pc-linux-gnu > > locale: > C > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] mouseexonpmcdf_1.1 affy_1.22.1 Biobase_2.4.1 > > loaded via a namespace (and not attached): > [1] affyio_1.12.0 preprocessCore_1.6.0 tools_2.9.2 > > > My CEL file can be found here: > > http://cs-analysis.clinmed.gla.ac.uk/MoEx/KC4MoEX1E143.CEL > > Peter > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > The University of Glasgow, charity number SC004401
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Dear Peter, Some time ago another Debian/Ubuntu user had the identical problem, which he solved as follows: https://stat.ethz.ch/pipermail/bioconductor/2009-August/029192.html However, this should not be the solution to the problem. I am testing xps on Mac, WinXP and OpenSUSE11.1, where compilation works w/o problem. When googling the error message I found the following site: http://ubuntuforums.org/showthread.php?t=165902 Thus, I assume that maybe your Ubuntu installation misses some development files, maybe the "glibc-headers" and "glibc-kernheaders", as mentioned in the link. However, please note that I am not an Ubuntu/Debian expert, thus maybe someone else could give you a better advice. BTW, which version of ROOT are you using? Did you compile it? Did you compile R? Best regards Christian Peter Saffrey wrote: > Hi, > > Thanks for the recommendation. This seems to bring its own problems. Here is the error from trying to install xps. > > g++ -O2 -Wall -fPIC -pthread -m64 -I/usr/include/root -c XPSUtils.cxx > XPSUtils.cxx: In function 'void READ_WSTRING(std::ifstream&, char*&, Bool_t)': > XPSUtils.cxx:4611: error: 'wcstombs' was not declared in this scope > make: *** [XPSUtils.o] Error 1 > ERROR: compilation failed for package 'xps' > > $ uname -a > Linux cs-analysis 2.6.31-17-generic #54-Ubuntu SMP Thu Dec 10 17:01:44 UTC 2009 x86_64 GNU/Linux > $ gcc --version > gcc (Ubuntu 4.4.1-4ubuntu9) 4.4.1 > > Cheers, > > Peter > > ________________________________________ > From: cstrato [cstrato at aon.at] > Sent: 08 February 2010 21:44 > To: Peter Saffrey > Cc: bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] Problem with MAS5 calls on MoEx array > > Dear Peter, > > Please note that exon arrays have only PM probesets and no MM probesets, > thus usually it is not possible to do mas5 with these arrays. > > As far as I know my BioC package "xps" is the only package where you can > indeed apply mas5 to exon arrays. > > Best regards > Christian > _._._._._._._._._._._._._._._._._._ > C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a > V.i.e.n.n.a A.u.s.t.r.i.a > e.m.a.i.l: cstrato at aon.at > _._._._._._._._._._._._._._._._._._ > > > Peter Saffrey wrote: > >> I am using the affy library with mouseexonpmcdf to compute RMA and MAS5 calls. >> The RMA works fine. The MAS5 throws errors: >> >> >> >>> library("affy") >>> >>> >> Loading required package: Biobase >> >> Welcome to Bioconductor >> >> Vignettes contain introductory material. To view, type >> 'openVignette()'. To cite Bioconductor, see >> 'citation("Biobase")' and for packages 'citation(pkgname)'. >> >> >> >>> adata <- ReadAffy() >>> adata at cdfName <- "mouseexonpmcdf" >>> pmaCalls <- mas5(adata) >>> >>> >> background correction: mas >> PM/MM correction : mas >> expression values: mas >> background correcting...Error in as.vector(data) : NA/NaN/Inf in foreign >> function call (arg 1) >> >> >>> sessionInfo() >>> >>> >> R version 2.9.2 (2009-08-24) >> x86_64-pc-linux-gnu >> >> locale: >> C >> >> attached base packages: >> [1] stats graphics grDevices utils datasets methods base >> >> other attached packages: >> [1] mouseexonpmcdf_1.1 affy_1.22.1 Biobase_2.4.1 >> >> loaded via a namespace (and not attached): >> [1] affyio_1.12.0 preprocessCore_1.6.0 tools_2.9.2 >> >> >> My CEL file can be found here: >> >> http://cs-analysis.clinmed.gla.ac.uk/MoEx/KC4MoEX1E143.CEL >> >> Peter >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >> >> >> > > > The University of Glasgow, charity number SC004401 > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > >
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Dear Peter, The problem turned out to be a missing "#include <cstdlib>" in some source files, which gcc-4.3.2 did accept, while gcc-4.4.1 is more restrictive. Thus I have uploaded a new version "xps-1.6.3" to BioC, which should solve the problem. Since I do not have gcc-4.4.1 and thus cannot test if the new version works, I would appreciate if you could test if xps-1.6.3 works on your system. Best regards Christian Peter Saffrey wrote: > Hi, > > Thanks for the recommendation. This seems to bring its own problems. Here is the error from trying to install xps. > > g++ -O2 -Wall -fPIC -pthread -m64 -I/usr/include/root -c XPSUtils.cxx > XPSUtils.cxx: In function 'void READ_WSTRING(std::ifstream&, char*&, Bool_t)': > XPSUtils.cxx:4611: error: 'wcstombs' was not declared in this scope > make: *** [XPSUtils.o] Error 1 > ERROR: compilation failed for package 'xps' > > $ uname -a > Linux cs-analysis 2.6.31-17-generic #54-Ubuntu SMP Thu Dec 10 17:01:44 UTC 2009 x86_64 GNU/Linux > $ gcc --version > gcc (Ubuntu 4.4.1-4ubuntu9) 4.4.1 > > Cheers, > > Peter > > ________________________________________ > From: cstrato [cstrato at aon.at] > Sent: 08 February 2010 21:44 > To: Peter Saffrey > Cc: bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] Problem with MAS5 calls on MoEx array > > Dear Peter, > > Please note that exon arrays have only PM probesets and no MM probesets, > thus usually it is not possible to do mas5 with these arrays. > > As far as I know my BioC package "xps" is the only package where you can > indeed apply mas5 to exon arrays. > > Best regards > Christian > _._._._._._._._._._._._._._._._._._ > C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a > V.i.e.n.n.a A.u.s.t.r.i.a > e.m.a.i.l: cstrato at aon.at > _._._._._._._._._._._._._._._._._._ > > > Peter Saffrey wrote: > >> I am using the affy library with mouseexonpmcdf to compute RMA and MAS5 calls. >> The RMA works fine. The MAS5 throws errors: >> >> >> >>> library("affy") >>> >>> >> Loading required package: Biobase >> >> Welcome to Bioconductor >> >> Vignettes contain introductory material. To view, type >> 'openVignette()'. To cite Bioconductor, see >> 'citation("Biobase")' and for packages 'citation(pkgname)'. >> >> >> >>> adata <- ReadAffy() >>> adata at cdfName <- "mouseexonpmcdf" >>> pmaCalls <- mas5(adata) >>> >>> >> background correction: mas >> PM/MM correction : mas >> expression values: mas >> background correcting...Error in as.vector(data) : NA/NaN/Inf in foreign >> function call (arg 1) >> >> >>> sessionInfo() >>> >>> >> R version 2.9.2 (2009-08-24) >> x86_64-pc-linux-gnu >> >> locale: >> C >> >> attached base packages: >> [1] stats graphics grDevices utils datasets methods base >> >> other attached packages: >> [1] mouseexonpmcdf_1.1 affy_1.22.1 Biobase_2.4.1 >> >> loaded via a namespace (and not attached): >> [1] affyio_1.12.0 preprocessCore_1.6.0 tools_2.9.2 >> >> >> My CEL file can be found here: >> >> http://cs-analysis.clinmed.gla.ac.uk/MoEx/KC4MoEX1E143.CEL >> >> Peter >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >> >> >> > > > The University of Glasgow, charity number SC004401 > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > >
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cstrato wrote: > The problem turned out to be a missing "#include <cstdlib>" in some > source files, which gcc-4.3.2 did accept, while gcc-4.4.1 is more > restrictive. Thus I have uploaded a new version "xps-1.6.3" to BioC, > which should solve the problem. > Yes, I tried the instructions you posted here: https://stat.ethz.ch/pipermail/bioconductor/2009-August/029192.html which essentially involving adding #include <cstdlib> in a few places, and this made the package compile. I couldn't install your package with biocLite() because it kept hitting the old version. If I install it from the tgz, I get: XPSPreProcessing.cxx:5821: error: call of overloaded 'abs(Int_t&)' is ambiguous Adding the #include <cstdlib> line in XPSPreProcessing.cxx fixed the problem, though - it now compiles with g++-4.4.1-4. Thanks, Peter
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cstrato ★ 3.9k
@cstrato-908
Last seen 6.2 years ago
Austria
Dear Peter, I am glad to hear that you could finally install xps. Thank you for mentioning the missing include file, so I have just uploaded "xps-1.6.4" to BioC. It will probably take 2-3 days until you can download the new version using biocLite(). Please let me know if you have any further questions. Best regards Christian Peter Saffrey wrote: > cstrato wrote: >> The problem turned out to be a missing "#include <cstdlib>" in some >> source files, which gcc-4.3.2 did accept, while gcc-4.4.1 is more >> restrictive. Thus I have uploaded a new version "xps-1.6.3" to BioC, >> which should solve the problem. >> > > Yes, I tried the instructions you posted here: > > https://stat.ethz.ch/pipermail/bioconductor/2009-August/029192.html > > which essentially involving adding #include <cstdlib> in a few places, > and this made the package compile. > > I couldn't install your package with biocLite() because it kept > hitting the old version. If I install it from the tgz, I get: > > XPSPreProcessing.cxx:5821: error: call of overloaded 'abs(Int_t&)' is > ambiguous > > Adding the #include <cstdlib> line in XPSPreProcessing.cxx fixed the > problem, though - it now compiles with g++-4.4.1-4. > > Thanks, > > Peter >
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