affy 10k
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@robinson-peter-1107
Last seen 10.3 years ago
Dear Listers, I would like to use the oligo package to do genotype calls for the affymetrix 10K chip. For some reason, I have not been able to find a package like "pd.mapping250k.sty", which is used for the 250K/Sty chip. In other words, I had used this code for the 250k chip: >biocLite("pd.mapping250k.sty") >library("oligo") >outputDir <- file.path(getwd(), "myGenotype") >fullFilenames <- list.files("myDirectory",pattern="CEL$", ignore.case="TRUE",full.names=TRUE) crlmm(fullFilenames, outputDir, verbose = TRUE) and I am not sure what annotation file to use for the 10K (if in fact there is one). Thanks for any tips! -Peter -- Dr. med. Peter N. Robinson, MSc. Institut f?r Medizinische Genetik Universit?tsklinikum Charite Humboldt-Universit?t Augustenburger Platz 1 13353 Berlin Germany voice: 49-30-450569124 fax: 49-30-450569915 email: peter.robinson at charite.de http://compbio.charite.de/ http://www.human-phenotype-ontology.org
Annotation oligo Annotation oligo • 1.1k views
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@vincent-j-carey-jr-4
Last seen 11 weeks ago
United States
My recollection is that we did not build for this chip because it was not in wide use. You should be able to build a pd.mapping* package using the pdInfoBuilder package. On Mon, Nov 2, 2009 at 9:22 AM, Peter Robinson <peter.robinson at="" charite.de=""> wrote: > Dear Listers, > > I would like to use the oligo package to do genotype calls for the > affymetrix 10K chip. For some reason, I have not been able to find a package > like "pd.mapping250k.sty", which is used for the 250K/Sty chip. > > In other words, I had used this code for the 250k chip: > >>biocLite("pd.mapping250k.sty") > > > >>library("oligo") >>outputDir <- file.path(getwd(), "myGenotype") >>fullFilenames <- list.files("myDirectory",pattern="CEL$", >> ignore.case="TRUE",full.names=TRUE) > crlmm(fullFilenames, outputDir, verbose = TRUE) > > > and I am not sure what annotation file to use for the 10K (if in fact there > is one). Thanks for any tips! > -Peter > > > -- > Dr. med. Peter N. Robinson, MSc. > Institut f?r Medizinische Genetik > Universit?tsklinikum Charite > Humboldt-Universit?t > Augustenburger Platz 1 > 13353 Berlin > Germany > voice: 49-30-450569124 > fax: ? 49-30-450569915 > email: peter.robinson at charite.de > http://compbio.charite.de/ > http://www.human-phenotype-ontology.org > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor >
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It has been pointed out to me that the main genotyping facilities for snp chips in bioconductor require availability of hapmap samples for calibration. As far as I know, the 10k chip was not applied to hapmap data, so neither snprma nor crlmm can work for this platform. On Mon, Nov 2, 2009 at 11:57 AM, Vincent Carey <stvjc at="" channing.harvard.edu=""> wrote: > My recollection is that we did not build for this chip because it was > not in wide use. ?You should be able to build a pd.mapping* package > using the pdInfoBuilder package. > > On Mon, Nov 2, 2009 at 9:22 AM, Peter Robinson > <peter.robinson at="" charite.de=""> wrote: >> Dear Listers, >> >> I would like to use the oligo package to do genotype calls for the >> affymetrix 10K chip. For some reason, I have not been able to find a package >> like "pd.mapping250k.sty", which is used for the 250K/Sty chip. >> >> In other words, I had used this code for the 250k chip: >> >>>biocLite("pd.mapping250k.sty") >> >> >> >>>library("oligo") >>>outputDir <- file.path(getwd(), "myGenotype") >>>fullFilenames <- list.files("myDirectory",pattern="CEL$", >>> ignore.case="TRUE",full.names=TRUE) >> crlmm(fullFilenames, outputDir, verbose = TRUE) >> >> >> and I am not sure what annotation file to use for the 10K (if in fact there >> is one). Thanks for any tips! >> -Peter >> >> >> -- >> Dr. med. Peter N. Robinson, MSc. >> Institut f?r Medizinische Genetik >> Universit?tsklinikum Charite >> Humboldt-Universit?t >> Augustenburger Platz 1 >> 13353 Berlin >> Germany >> voice: 49-30-450569124 >> fax: ? 49-30-450569915 >> email: peter.robinson at charite.de >> http://compbio.charite.de/ >> http://www.human-phenotype-ontology.org >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> >
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Unfortunately, that won't be sufficient (not for genotyping). One reason is that 10k is not in wide use indeed. But, also, to the best of our knowledge, there is not training data on that platform (HapMap samples and genotype calls), which would allow us to estimate the parameters needed by CRLMM. b On Nov 2, 2009, at 2:57 PM, Vincent Carey wrote: > My recollection is that we did not build for this chip because it was > not in wide use. You should be able to build a pd.mapping* package > using the pdInfoBuilder package. > > On Mon, Nov 2, 2009 at 9:22 AM, Peter Robinson > <peter.robinson at="" charite.de=""> wrote: >> Dear Listers, >> >> I would like to use the oligo package to do genotype calls for the >> affymetrix 10K chip. For some reason, I have not been able to find >> a package >> like "pd.mapping250k.sty", which is used for the 250K/Sty chip. >> >> In other words, I had used this code for the 250k chip: >> >>> biocLite("pd.mapping250k.sty") >> >> >> >>> library("oligo") >>> outputDir <- file.path(getwd(), "myGenotype") >>> fullFilenames <- list.files("myDirectory",pattern="CEL$", >>> ignore.case="TRUE",full.names=TRUE) >> crlmm(fullFilenames, outputDir, verbose = TRUE) >> >> >> and I am not sure what annotation file to use for the 10K (if in >> fact there >> is one). Thanks for any tips! >> -Peter >> >> >> -- >> Dr. med. Peter N. Robinson, MSc. >> Institut f?r Medizinische Genetik >> Universit?tsklinikum Charite >> Humboldt-Universit?t >> Augustenburger Platz 1 >> 13353 Berlin >> Germany >> voice: 49-30-450569124 >> fax: 49-30-450569915 >> email: peter.robinson at charite.de >> http://compbio.charite.de/ >> http://www.human-phenotype-ontology.org >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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For the record, there are two *different* Affymetrix 10K chips: Mapping10K_Xba131 and Mapping10K_Xba142. They are not the same (physical chip types): http://groups.google.com/group/aroma-affymetrix/web/mapping10k-xba131 http://groups.google.com/group/aroma-affymetrix/web/mapping10k-xba142 I think dChip do genotyping on both of these, but I might be wrong. My $.02 /Henrik On Mon, Nov 2, 2009 at 9:04 AM, Benilton Carvalho <bcarvalh at="" jhsph.edu=""> wrote: > Unfortunately, that won't be sufficient (not for genotyping). > > One reason is that 10k is not in wide use indeed. But, also, to the best of > our knowledge, there is not training data on that platform (HapMap samples > and genotype calls), which would allow us to estimate the parameters needed > by CRLMM. > > b > > On Nov 2, 2009, at 2:57 PM, Vincent Carey wrote: > >> My recollection is that we did not build for this chip because it was >> not in wide use. ?You should be able to build a pd.mapping* package >> using the pdInfoBuilder package. >> >> On Mon, Nov 2, 2009 at 9:22 AM, Peter Robinson >> <peter.robinson at="" charite.de=""> wrote: >>> >>> Dear Listers, >>> >>> I would like to use the oligo package to do genotype calls for the >>> affymetrix 10K chip. For some reason, I have not been able to find a >>> package >>> like "pd.mapping250k.sty", which is used for the 250K/Sty chip. >>> >>> In other words, I had used this code for the 250k chip: >>> >>>> biocLite("pd.mapping250k.sty") >>> >>> >>> >>>> library("oligo") >>>> outputDir <- file.path(getwd(), "myGenotype") >>>> fullFilenames <- list.files("myDirectory",pattern="CEL$", >>>> ignore.case="TRUE",full.names=TRUE) >>> >>> crlmm(fullFilenames, outputDir, verbose = TRUE) >>> >>> >>> and I am not sure what annotation file to use for the 10K (if in fact >>> there >>> is one). Thanks for any tips! >>> -Peter >>> >>> >>> -- >>> Dr. med. Peter N. Robinson, MSc. >>> Institut f?r Medizinische Genetik >>> Universit?tsklinikum Charite >>> Humboldt-Universit?t >>> Augustenburger Platz 1 >>> 13353 Berlin >>> Germany >>> voice: 49-30-450569124 >>> fax: ? 49-30-450569915 >>> email: peter.robinson at charite.de >>> http://compbio.charite.de/ >>> http://www.human-phenotype-ontology.org >>> >>> _______________________________________________ >>> Bioconductor mailing list >>> Bioconductor at stat.math.ethz.ch >>> https://stat.ethz.ch/mailman/listinfo/bioconductor >>> Search the archives: >>> http://news.gmane.org/gmane.science.biology.informatics.conductor >>> >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor >
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