[marrayNorm] Is it possible to obtain normalized intensity values ?
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@marcelo-luiz-de-laia-377
Last seen 10.2 years ago
Hi All, I would like to get normalized intensity values from a marrayNorm object. Is it possible? Regards Marcelo Luiz de Laia, M.Sc. Dep. de Tecnologia, Lab. Bioqu?mica e de Biologia Molecular Universidade Estadual Paulista - UNESP Via de Acesso Prof. Paulo Donato Castelane, Km 05 14.884-900 - Jaboticabal, SP, Brazil PhoneFax: 16 3209-2675/2676/2677 R. 202/208/203 (trab.) Phone res: 16 3203 2328 - www.lbm.fcav.unesp.br - mlaia@yahoo.com ---
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@jean-yee-hwa-yang-104
Last seen 10.2 years ago
Hi, data is a marrayNorm object M <- maM(data) gives you the normlized log-ratios A <- maA(data) gives you the average log-intensity You can solve the equation M = R - G A = 1/2(R + G) if you want to obtain the normalized log-intensity R and G. Cheers Jean On Tue, 11 Nov 2003, Marcelo Luiz de Laia wrote: > Hi All, > > I would like to get normalized intensity values from a marrayNorm object. > > Is it possible? > > Regards > > Marcelo Luiz de Laia, M.Sc. > Dep. de Tecnologia, Lab. BioquĂ­mica e de Biologia Molecular > Universidade Estadual Paulista - UNESP > Via de Acesso Prof. Paulo Donato Castelane, Km 05 > 14.884-900 - Jaboticabal, SP, Brazil > PhoneFax: 16 3209-2675/2676/2677 R. 202/208/203 (trab.) > Phone res: 16 3203 2328 - www.lbm.fcav.unesp.br - mlaia@yahoo.com > --- > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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To backsolve those equations then for normalized R and G, the normalized R = 0.5*(M-Mpred) + 1*A with normalized G = -0.5*(M-Mpred) + 1*A where Mpred is the predicted M from the Loess fit. Robert J. Tempelman Department of Animal Science 1205 Anthony Hall Michigan State University East Lansing, MI 48824-1225 Phone (517) 355-8445 Fax (517) 353-1699 email: tempelma@msu.edu ? ? > -----Original Message----- > From: bioconductor-bounces@stat.math.ethz.ch [mailto:bioconductor- > bounces@stat.math.ethz.ch] On Behalf Of Jean Yee Hwa Yang > Sent: Tuesday, November 11, 2003 4:41 PM > To: Marcelo Luiz de Laia > Cc: Bioconductor > Subject: Re: [BioC] [marrayNorm] Is it possible to obtain normalized > intensityvalues ? > > Hi, > > data is a marrayNorm object > M <- maM(data) gives you the normlized log-ratios > A <- maA(data) gives you the average log-intensity > You can solve the equation > M = R - G > A = 1/2(R + G) > > if you want to obtain the normalized log-intensity R and G. > > Cheers > > Jean > > > On Tue, 11 Nov 2003, Marcelo Luiz de Laia wrote: > > > Hi All, > > > > I would like to get normalized intensity values from a marrayNorm > object. > > > > Is it possible? > > > > Regards > > > > Marcelo Luiz de Laia, M.Sc. > > Dep. de Tecnologia, Lab. Bioqu?mica e de Biologia Molecular > > Universidade Estadual Paulista - UNESP > > Via de Acesso Prof. Paulo Donato Castelane, Km 05 > > 14.884-900 - Jaboticabal, SP, Brazil > > PhoneFax: 16 3209-2675/2676/2677 R. 202/208/203 (trab.) > > Phone res: 16 3203 2328 - www.lbm.fcav.unesp.br - mlaia@yahoo.com > > --- > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor
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