Hello, On the same topic of GenePix I had to create the targets files from a file provided by arrayexpress which looked something like this (I've deleted some cols to proceed with the example): SlideNumber Source Name Label FileName 1 8CT Cy5 1348628744.txt 2 5Exp Cy3 1348628729.txt 3 10Exp Cy5 1348628754.txt 4 4CT Cy3 1348628724.txt 5 1Exp Cy5 1348628709.txt 6 12Exp Cy5 1348628764.txt 7 6Exp Cy5 1348628734.txt 8 3CT Cy3 1348628719.txt 9 13Exp Cy3 1348628769.txt 10 7Exp Cy3 1348628739.txt 11 2CT Cy5 1348628714.txt 12 11Exp Cy3 1348628759.txt 13 9Exp Cy5 1348628749.txt 14 14CT Cy3 1348628774.txt Therefore I've created a target file that looks like this SlideNumber Filename Cy3 Cy5 1 1348628744.txt case control 2 1348628729.txt case control 3 1348628754.txt control case 4 1348628724.txt control case 5 1348628709.txt control case 6 1348628764.txt control case 7 1348628734.txt control case 8 1348628719.txt control case 9 1348628769.txt case control 10 1348628739.txt case control 11 1348628714.txt case control 12 1348628759.txt case control 13 1348628749.txt control case 14 1348628774.txt control case Is this correct? Luana _________________________________________________________________ Insert movie times and more without leaving Hotmail®. orial_QuickAdd_062009 [[alternative HTML version deleted]]