Hi, Does anybody know how to merge replicate spots in R Bioconductor? I use home-made spotted arrays and every probe is printed in triplicate. I normalize my data using limma package and I do not know how to get mean from these three replicates. Regards, Barbara Cegielska

Barbara, The function, duplicateCorrelation, which is discussed in the Limma manual can do this task. Best wishes, Rich ------------------------------------------------------------ Richard A. Friedman, PhD Associate Research Scientist, Biomedical Informatics Shared Resource Herbert Irving Comprehensive Cancer Center (HICCC) Lecturer, Department of Biomedical Informatics (DBMI) Educational Coordinator, Center for Computational Biology and Bioinformatics (C2B2)/ National Center for Multiscale Analysis of Genomic Networks (MAGNet) Room 824 Irving Cancer Research Center Columbia University 1130 St. Nicholas Ave New York, NY 10032 (212)851-4765 (voice) friedman at cancercenter.columbia.edu http://cancercenter.columbia.edu/~friedman/ In Memoriam, Thomas Disch On May 20, 2009, at 8:38 AM, Barbara Cegielska wrote: > Hi, > Does anybody know how to merge replicate spots in R Bioconductor? I > use home-made spotted arrays and every probe is printed in > triplicate. I normalize my data using limma package and I do not > know how to get mean from these three replicates. > > Regards, > Barbara Cegielska > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/ > gmane.science.biology.informatics.conductor