Hi all, I am using the microRNA Agilent platform to conduct a differential expression analysis. Does anyone know what are the processing protocol that is recommended by the Agilent manufacturer? I think that we should use the Total Gene Signal (TGS), but, does Agilent recommend any sort of normalization between arrays using the TGS, or the Agilent's recommendation is just to compare the TGS between the experimental conditions without doing anything else? Does anyone know if there is a sort of Agilent document where all of this is explained? Thanks a lot. pedro [[alternative HTML version deleted]]