PDNN for affy.
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@stephen-nyangoma-366
Last seen 10.2 years ago
Hi all, I am implementing the R for PDNN model by Laurent in R-1.7.1. The function find.params.pdnn works ok and I can obtain the Bs, Ns, Fs and the lambdas. I want to use equation 1 in the paper Li Zhang, Michael F. Miles and Kenneth D. Aldape - A model of molecular interactions on short oligonucleotide arrays, 2003, Nature Biotech., vol. 21, n.7 to obtain the fitted values. At the moment, I cannot see how to obtain the Es (especially the E^*, the average free energy for NSB). Does anyone have an idea of how to generate these values? Regards. Stephen.
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Laurent Gautier ★ 2.3k
@laurent-gautier-29
Last seen 10.2 years ago
Hi Stephen, Note that the pack 'affypdnn' was developped under R-1.7.1, but it should work out-of-the-box with R-1.8.0 and/or the latest devel version of 'affy'. I shall see if we can cram a short moment to include a function to do that before the end of the week... L. On Mon, Oct 13, 2003 at 10:01:55AM +0200, Stephen Nyangoma wrote: > Hi all, > I am implementing the R for PDNN model by Laurent in R-1.7.1. The > function find.params.pdnn works ok and I can obtain the Bs, Ns, Fs and > the lambdas. I want to use equation 1 in the paper > > Li Zhang, Michael F. Miles and Kenneth D. Aldape - > A model of molecular interactions on short oligonucleotide > arrays, 2003, Nature Biotech., vol. 21, n.7 > > to obtain the fitted values. At the moment, I cannot see how to obtain > the Es (especially the E^*, the average free energy for NSB). Does > anyone have an idea of how to generate these values? > > Regards. Stephen. > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor -- -------------------------------------------------------------- Laurent Gautier CBS, Building 208, DTU PhD. Student DK-2800 Lyngby,Denmark tel: +45 45 25 24 89 http://www.cbs.dtu.dk/laurent
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Thanks, Laurent for a quick reply. Stephen. On Mon, 2003-10-13 at 10:36, Laurent Gautier wrote: > Hi Stephen, > > Note that the pack 'affypdnn' was developped under R-1.7.1, > but it should work out-of-the-box with R-1.8.0 and/or > the latest devel version of 'affy'. > > I shall see if we can cram a short moment to include a function > to do that before the end of the week... > > L. > > > On Mon, Oct 13, 2003 at 10:01:55AM +0200, Stephen Nyangoma wrote: > > Hi all, > > I am implementing the R for PDNN model by Laurent in R-1.7.1. The > > function find.params.pdnn works ok and I can obtain the Bs, Ns, Fs and > > the lambdas. I want to use equation 1 in the paper > > > > Li Zhang, Michael F. Miles and Kenneth D. Aldape - > > A model of molecular interactions on short oligonucleotide > > arrays, 2003, Nature Biotech., vol. 21, n.7 > > > > to obtain the fitted values. At the moment, I cannot see how to obtain > > the Es (especially the E^*, the average free energy for NSB). Does > > anyone have an idea of how to generate these values? > > > > Regards. Stephen. > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > -- > -------------------------------------------------------------- > Laurent Gautier CBS, Building 208, DTU > PhD. Student DK-2800 Lyngby,Denmark > tel: +45 45 25 24 89 http://www.cbs.dtu.dk/laurent
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Dear all, I have a problem with Bioconductor packages, and in particular affy, after updating R to 1.8.0 on a Linux\Debian box I reinstalled all Bioconductor packages but I get this errors: >library(affy) Error in bindingIsLocked(m2,where): not an environment (repeated twice) Error in getClass(Class, where=topenv(parent.frame())): "MIAME" is not a defined class Error in library(affy) : .First.lib failed Any suggestion? TIA, Ste -- Stefano Calza, Sezione di Statistica Medica Dip. di Scienze Biomediche e Biotecnologie Universit? degli Studi di Brescia - Italy Viale Europa, 11 25123 Brescia email: calza@med.unibs.it Telefono/Phone: +390303717532 Fax: +390303701157
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