Hi all, How do I get my normalised Illumina data , via bead array, exported into a expression set as a tab delimited .txt file ? I have tried following the notes on the bioconductor website and anything else I can find on the web. I have also looked through previous posts and I have no been able to find the answer. I am not use to beadarray or any command line type analysis. Thanks you so much for you help. Matt Here is my code: (running R2.7.2, and the latest version of beadarray) *library(limma)* *library(beadarray) **BSData = readBeadSummaryData("expset-all.txt", qcFile=NULL, sampleSheet=NULL,sep="\t",skip=8, ProbeID="PROBE_ID", columns = list(exprs = "AVG_Signal", se.exprs="BEAD_STDERR", NoBeads = "Avg_NBEADS", Detection="Detection Pval"), dec=".") **slotNames(BSData) **names(assayData(BSData))* *BSData.log2.qspline = normaliseIllumina(BSData, method="qspline", transform="log2")* *library(convert) eSet = new("ExpressionSet", assayData= exprs(BSData.log2.qspline$AVG_Signal)) *Error in function (classes, fdef, mtable) : unable to find an inherited method for function "exprs", for signature "NULL" What is wrong with this final above comand that causes the error? -- Matthew Vitalone B.Sc (Hons) PhD(progress) NHMRC Centre for Clinical Research Excellence in Renal Medicine Centre For Transplant and Renal Research Transplant Laboratory (Room 2175), Clinical Sciences Westmead Millennium Institute Darcy Road, Westmead, NSW, 2145. Sydney, Australia. Phone: (+61-2) 9845 8906 Mobile: 0416 041783 Hosp. Page: 27147 Fax: (+61-2) 9633 9351 Email: matthew_vitalone at wmi.usyd.edu.au _______________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential infor...{{dropped:10}}