cellHTS2 - 2 channel batch analysis
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Steve Taylor ▴ 280
@steve-taylor-2838
Last seen 10.2 years ago
Hi, I am trying to set up a two channel analysis using cellHTS2. I have cell line X. X has control (C) and experiment (E) conditions. The screen uses 3 x 96 well plates and C and E have 2 replicates each, hence C and E have 6 plates each in total. It gets complicated because there is a repeat of the whole experiment (on a separate day) and the experimenter used a different plate configuration. Also 3 of the control plates were not run, so for the second experimental run C has 3 plates and E has 6 plates. I'd like to combine both experiments but wondered what is the best way? I read you can normalize for batch effects which looks like it should help but on the whole I am unsure how to configure this analysis. Thanks for any help, Steve ------------------------------------------------------------------ Medical Sciences Division Weatherall Institute of Molecular Medicine/Sir William Dunn School Oxford University
cellHTS2 cellHTS2 • 865 views
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