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Steve Taylor
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280
@steve-taylor-2838
Last seen 10.2 years ago
Hi,
I am trying to set up a two channel analysis using cellHTS2.
I have cell line X. X has control (C) and experiment (E) conditions.
The screen uses 3 x 96 well plates and C and E have 2 replicates each,
hence C and E have 6 plates each in total.
It gets complicated because there is a repeat of the whole experiment
(on a separate day) and the experimenter used a different plate
configuration. Also 3 of the control plates were not run, so for
the second experimental run C has 3 plates and E has 6 plates.
I'd like to combine both experiments but wondered what is the best
way? I read you can normalize for batch effects which looks like it
should help but on the whole I am unsure how to configure this
analysis.
Thanks for any help,
Steve
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Medical Sciences Division
Weatherall Institute of Molecular Medicine/Sir William Dunn School
Oxford University