Entering edit mode
Elliot Kleiman
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150
@elliot-kleiman-2565
Last seen 10.2 years ago
Hi BioC,
[Question]:
* How do you convert Illumina ProbeIDs to nuIDs without nuID
indexed annotation libraries?
i.e.,
... the following previous BioC 2.1 released nuID indexed annotation
libraries,
* lumiRatV1
* lumiMouseV1
* lumiHumanV1
* lumiHumanV2
... are not available in BioC release 2.2,
* http://www.bioconductor.org/packages/2.2/data/annotation/
RE: My objective is to map probeIDs to nuIDs standalone without
first having to create a lumiBatch object via the lumi package.
There is a new annotation package, 'lumiRatAll.db' in BioC 2.2,
but it doesn't provide the functional mapping of ProbeIDs to nuIDs;
it assumes you already have nuIDs.
e.g.,
The lumi package offers a function that can map Illumina probeIDs
to nuIDs, 'probeID2nuID()'. But the problem is that it requires
a 'lib' argument to specify a nuID indexed annotation library
(none of which are currently available in the current 2.2 release).
* http://www.bioconductor.org/packages/2.2/data/annotation/
e.g.,
# load lumi package from BioC release 2.2
> library(lumi)
# get help on lumi function probeID2nuID
> help(probeID2nuID)
Print-out excerpt of help documentation for function
'probeID2nuID()' from package 'lumi',
# probeID2nuID package:lumi R Documentation
# Usage:
# probeID2nuID(probeID, lib = "lumiHumanV1")
# Arguments:
# probeID: a vector of Illumina ProbeID
# lib: a nuID indexed Illumina annotation library
e.g.
# Not Run b/c no annotation libs loaded b/c they aren't available to
install
> nu <- probeID2nuID(pid, lib = "lumiHumanV1")
Am I [like totally] missing something here?
Thanks,
... Elliot
> # print sessionInfo()
> sessionInfo()
R version 2.7.2 (2008-08-25)
i686-pc-linux-gnu
locale:
LC_CTYPE=en_US;LC_NUMERIC=C;LC_TIME=en_US;LC_COLLATE=C;LC_MONETARY=C;L
C_MESSAGES=en_US;LC_PAPER=en_US;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;
LC_MEASUREMENT=en_US;LC_IDENTIFICATION=C
attached base packages:
[1] splines tools stats graphics grDevices utils
datasets
[8] methods base
other attached packages:
[1] illuminaRatv1ProbeID.db_1.1.1 GOstats_2.6.0
[3] Category_2.6.0 genefilter_1.20.0
[5] survival_2.34-1 RBGL_1.16.0
[7] GO.db_2.2.0 graph_1.18.1
[9] lumiRatAll.db_1.2.0 lumi_1.6.2
[11] mgcv_1.4-1 beadarray_1.8.0
[13] affy_1.18.2 preprocessCore_1.2.1
[15] affyio_1.8.1 geneplotter_1.18.0
[17] annotate_1.18.0 xtable_1.5-3
[19] AnnotationDbi_1.2.2 RSQLite_0.7-0
[21] DBI_0.2-4 lattice_0.17-14
[23] Biobase_2.0.1 limma_2.14.6
loaded via a namespace (and not attached):
[1] KernSmooth_2.22-22 RColorBrewer_1.0-2 cluster_1.11.11
grid_2.7.2
[5] tcltk_2.7.2
--
__________________________
MS graduate student
Program in Computational Science
San Diego State University
http://www.csrc.sdsu.edu/
http://www.rohan.sdsu.edu/~kleiman/