lumi, lumiHumanV2 and GOstats
1
0
Entering edit mode
Al Ivens ▴ 270
@al-ivens-1646
Last seen 10.2 years ago
Hi all, I have been trying out the GOstats section of the lumi vignette, using data obtained from Illumina WG6 chips. I use the lumiHumanV2 package. I have two installations of R and Bioconductor: 1. On my Windoze laptop, I have R 2.6.2 and as far as I am aware, up to date versions of the relevant Bioconductor packages for 2.6.2. Doing the GOstats works just fine. 2. On my Unix server, I have R 2.7.0, and as far as I am aware, the most up to date Bioc packages. Doing the GOstats on this platform, using the same GOstats script etc, doesn't work. Below is the error, traceback and sessionInfo. At a bit of loss as to why the difference! Cheers and thanks in advance for any assistance, a X--------------------------------------------------------- Error in do.call(paste(pname, "dbconn", sep = "_"), list()) : could not find function "lumiHumanV2_dbconn" > > > traceback() 8: do.call(paste(pname, "dbconn", sep = "_"), list()) 7: getGoToEntrezMap_db(p) 6: categoryToEntrezBuilder(p) 5: categoryToEntrezBuilder(p) 4: is(object, Cl) 3: is(object, Cl) 2: .valueClassTest(standardGeneric("hyperGTest"), "HyperGResultBase", "hyperGTest") 1: hyperGTest(BPparams) > > sessionInfo() R version 2.7.0 (2008-04-22) x86_64-pc-linux-gnu locale: LC_CTYPE=en_GB.UTF-8;LC_NUMERIC=C;LC_TIME=en_GB.UTF-8;LC_COLLATE=en_GB .U TF-8;LC_MONETARY=C;LC_MESSAGES=en_GB.UTF-8;LC_PAPER=en_GB.UTF-8;LC_NAM E= C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_GB.UTF-8;LC_IDENTIFICA TI ON=C attached base packages: [1] grid splines tools stats graphics grDevices utils [8] datasets methods base other attached packages: [1] arrayQualityMetrics_1.6.0 beadarray_1.8.0 [3] latticeExtra_0.3-1 simpleaffy_2.16.0 [5] affyPLM_1.16.0 gcrma_2.12.0 [7] matchprobes_1.12.0 RColorBrewer_1.0-2 [9] vsn_3.6.0 geneplotter_1.18.0 [11] lattice_0.17-2 gplots_2.6.0 [13] gdata_2.4.1 gtools_2.4.0 [15] limma_2.14.1 GOstats_2.6.0 [17] Category_2.6.0 genefilter_1.20.0 [19] survival_2.34 RBGL_1.16.0 [21] annotate_1.18.0 xtable_1.5-2 [23] GO.db_2.2.0 AnnotationDbi_1.2.1 [25] RSQLite_0.6-8 DBI_0.2-4 [27] graph_1.18.1 lumiHumanV2_1.3.1 [29] lumi_1.6.0 mgcv_1.3-31 [31] affy_1.18.0 preprocessCore_1.2.0 [33] affyio_1.8.0 Biobase_2.0.1 loaded via a namespace (and not attached): [1] cluster_1.11.9 KernSmooth_2.22-21 -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE.
GO lumiHumanV2 GOstats lumi GO lumiHumanV2 GOstats lumi • 1.1k views
ADD COMMENT
0
Entering edit mode
@james-w-macdonald-5106
Last seen just now
United States
Hi Al, Al Ivens wrote: > Hi all, > > I have been trying out the GOstats section of the lumi vignette, using > data obtained from Illumina WG6 chips. I use the lumiHumanV2 package. > > I have two installations of R and Bioconductor: > > 1. On my Windoze laptop, I have R 2.6.2 and as far as I am aware, up to > date versions of the relevant Bioconductor packages for 2.6.2. Doing > the GOstats works just fine. > 2. On my Unix server, I have R 2.7.0, and as far as I am aware, the most > up to date Bioc packages. Doing the GOstats on this platform, using the > same GOstats script etc, doesn't work. > > Below is the error, traceback and sessionInfo. At a bit of loss as to > why the difference! > > Cheers and thanks in advance for any assistance, > > a > > X--------------------------------------------------------- > Error in do.call(paste(pname, "dbconn", sep = "_"), list()) : > could not find function "lumiHumanV2_dbconn" This is because there isn't a lumiHumanV2 in BioC 2.2. Not sure how you got this installed, as biocLite() shouldn't have done so. Anyway, I believe you want the lumiHumanAll.db package (and to remove the lumiHumanV2 package, as that is from an older BioC release). Best, Jim >> >> traceback() > 8: do.call(paste(pname, "dbconn", sep = "_"), list()) > 7: getGoToEntrezMap_db(p) > 6: categoryToEntrezBuilder(p) > 5: categoryToEntrezBuilder(p) > 4: is(object, Cl) > 3: is(object, Cl) > 2: .valueClassTest(standardGeneric("hyperGTest"), "HyperGResultBase", > "hyperGTest") > 1: hyperGTest(BPparams) >> sessionInfo() > R version 2.7.0 (2008-04-22) > x86_64-pc-linux-gnu > > locale: > LC_CTYPE=en_GB.UTF-8;LC_NUMERIC=C;LC_TIME=en_GB.UTF-8;LC_COLLATE=en_ GB.U > TF-8;LC_MONETARY=C;LC_MESSAGES=en_GB.UTF-8;LC_PAPER=en_GB.UTF-8;LC_N AME= > C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_GB.UTF-8;LC_IDENTIFI CATI > ON=C > > attached base packages: > [1] grid splines tools stats graphics grDevices utils > > [8] datasets methods base > > other attached packages: > [1] arrayQualityMetrics_1.6.0 beadarray_1.8.0 > [3] latticeExtra_0.3-1 simpleaffy_2.16.0 > [5] affyPLM_1.16.0 gcrma_2.12.0 > [7] matchprobes_1.12.0 RColorBrewer_1.0-2 > [9] vsn_3.6.0 geneplotter_1.18.0 > [11] lattice_0.17-2 gplots_2.6.0 > [13] gdata_2.4.1 gtools_2.4.0 > [15] limma_2.14.1 GOstats_2.6.0 > [17] Category_2.6.0 genefilter_1.20.0 > [19] survival_2.34 RBGL_1.16.0 > [21] annotate_1.18.0 xtable_1.5-2 > [23] GO.db_2.2.0 AnnotationDbi_1.2.1 > [25] RSQLite_0.6-8 DBI_0.2-4 > [27] graph_1.18.1 lumiHumanV2_1.3.1 > [29] lumi_1.6.0 mgcv_1.3-31 > [31] affy_1.18.0 preprocessCore_1.2.0 > [33] affyio_1.8.0 Biobase_2.0.1 > > loaded via a namespace (and not attached): > [1] cluster_1.11.9 KernSmooth_2.22-21 > > > > -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
ADD COMMENT
0
Entering edit mode
Hi Jim, Ah. That explains it, many thanks. And no, biocLite wasnt guilty! Cheers, a > -----Original Message----- > From: James W. MacDonald [mailto:jmacdon at med.umich.edu] > Sent: 29 May 2008 14:44 > To: Al Ivens > Cc: 'bioc' > Subject: Re: [BioC] lumi, lumiHumanV2 and GOstats > > > Hi Al, > > Al Ivens wrote: > > Hi all, > > > > I have been trying out the GOstats section of the lumi > vignette, using > > data obtained from Illumina WG6 chips. I use the > lumiHumanV2 package. > > > > I have two installations of R and Bioconductor: > > > > 1. On my Windoze laptop, I have R 2.6.2 and as far as I am > aware, up > > to date versions of the relevant Bioconductor packages for 2.6.2. > > Doing the GOstats works just fine. 2. On my Unix server, I have R > > 2.7.0, and as far as I am aware, the most up to date Bioc > packages. > > Doing the GOstats on this platform, using the same GOstats > script etc, > > doesn't work. > > > > Below is the error, traceback and sessionInfo. At a bit of > loss as to > > why the difference! > > > > Cheers and thanks in advance for any assistance, > > > > a > > > > X--------------------------------------------------------- > > Error in do.call(paste(pname, "dbconn", sep = "_"), list()) : > > could not find function "lumiHumanV2_dbconn" > > This is because there isn't a lumiHumanV2 in BioC 2.2. Not > sure how you > got this installed, as biocLite() shouldn't have done so. > > Anyway, I believe you want the lumiHumanAll.db package (and to remove > the lumiHumanV2 package, as that is from an older BioC release). > > Best, > > Jim > > > >> > >> traceback() > > 8: do.call(paste(pname, "dbconn", sep = "_"), list()) > > 7: getGoToEntrezMap_db(p) > > 6: categoryToEntrezBuilder(p) > > 5: categoryToEntrezBuilder(p) > > 4: is(object, Cl) > > 3: is(object, Cl) > > 2: .valueClassTest(standardGeneric("hyperGTest"), > "HyperGResultBase", > > "hyperGTest") > > 1: hyperGTest(BPparams) > >> sessionInfo() > > R version 2.7.0 (2008-04-22) > > x86_64-pc-linux-gnu > > > > locale: > > > LC_CTYPE=en_GB.UTF-8;LC_NUMERIC=C;LC_TIME=en_GB.UTF-8;LC_COLLATE=en_GB > > .U > > > TF-8;LC_MONETARY=C;LC_MESSAGES=en_GB.UTF-8;LC_PAPER=en_GB.UTF- > 8;LC_NAME= > > > C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_GB.UTF-8;LC_ID > ENTIFICATI > > ON=C > > > > attached base packages: > > [1] grid splines tools stats graphics > grDevices utils > > > > [8] datasets methods base > > > > other attached packages: > > [1] arrayQualityMetrics_1.6.0 beadarray_1.8.0 > > [3] latticeExtra_0.3-1 simpleaffy_2.16.0 > > [5] affyPLM_1.16.0 gcrma_2.12.0 > > [7] matchprobes_1.12.0 RColorBrewer_1.0-2 > > [9] vsn_3.6.0 geneplotter_1.18.0 > > [11] lattice_0.17-2 gplots_2.6.0 > > [13] gdata_2.4.1 gtools_2.4.0 > > [15] limma_2.14.1 GOstats_2.6.0 > > [17] Category_2.6.0 genefilter_1.20.0 > > [19] survival_2.34 RBGL_1.16.0 > > [21] annotate_1.18.0 xtable_1.5-2 > > [23] GO.db_2.2.0 AnnotationDbi_1.2.1 > > [25] RSQLite_0.6-8 DBI_0.2-4 > > [27] graph_1.18.1 lumiHumanV2_1.3.1 > > [29] lumi_1.6.0 mgcv_1.3-31 > > [31] affy_1.18.0 preprocessCore_1.2.0 > > [33] affyio_1.8.0 Biobase_2.0.1 > > > > loaded via a namespace (and not attached): > > [1] cluster_1.11.9 KernSmooth_2.22-21 > > > > > > > > > > -- > James W. MacDonald, M.S. > Biostatistician > Affymetrix and cDNA Microarray Core > University of Michigan Cancer Center > 1500 E. Medical Center Drive > 7410 CCGC > Ann Arbor MI 48109 > 734-647-5623 > -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969, whose registered office is 215 Euston Road, London, NW1 2BE.
ADD REPLY

Login before adding your answer.

Traffic: 797 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6