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Liolios, Konstantinos [BSD] - MED
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30
@liolios-konstantinos-bsd-med-2786
Last seen 10.3 years ago
Dear bioconductors,
I have been given Exiqon miRCURY(tm) LNA Array (miRNA) expression gpr
files in excel format. They are single channel so they are actually
missing all 635 related (mean/median/back/for etc.) columns. I
believe
vsn is the way to normalize these data but I can not find anywhere how
to import such a file. Most tutorials use data that already come with
the package or assume you have .cel files and an affymetrix annotation
file. I have used read.table which creates a data.frame but most
normalization packages require you to have your data as an expression
set. I was wondering if somebody knows how to import single channel
gpr
files or how to manually create an expression set from a data.frame
w/o
an annotation file.
Best regards
Dinos
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