Affy data
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Kathy Duncan ▴ 130
@kathy-duncan-2722
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@james-w-macdonald-5106
Last seen 2 days ago
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Hi Kathy, The different chip types will have to be processed and analyzed separately. The easiest way to do so is to make separate subdirectories and put the different chip types in their own directory. Best, Jim Kathy Duncan wrote: > Hi All, > > I've got an Affymetrix dataset which I need to process and finally get the > list of genes and their respective expression levels. Without having much > prior idea and experience, I tried the ReadAffy() function within the > directory containing the .CEL files and I came across the error saying : > > Error in read.affybatch(filenames = l$filenames, phenoData = l$phenoData, : > > Cel file MN.CEL does not seem to have the correct dimensions > > Then I tried the following command for each individual .CEL files of that > investigation (as I am not sure how to get such information about all the > files using a single command): > > whatcdf ("filename", compress = getOption("BioC")$affy$compress.cel) > > I could see that the investigation is referring to different CDF files. > > Could you please inform me how I should proceed ! > > kathy > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, MS Biostatistician UMCCC cDNA and Affymetrix Core University of Michigan 1500 E Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
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Hi Kathy, Assuming the chips are all the same species, I would probably use something like MergeMaid. There may be others -- you can check here: http://bioconductor.org/packages/2.2/DifferentialExpression.html Best, Jim Kathy Duncan wrote: > Hi Jim, > > Thanks ! > > Further, I'm eagerly looking forward to comment(s) on the methods of > integrating those separately processed and analyzed data, as you have > mentioned. > [Else, pointers towards some ref. would also be helpful] > > kathy > > > > > > On Sat, Apr 12, 2008 at 1:38 AM, James MacDonald <jmacdon at="" med.umich.edu=""> <mailto:jmacdon at="" med.umich.edu="">> wrote: > > Hi Kathy, > > The different chip types will have to be processed and analyzed > separately. The easiest way to do so is to make separate > subdirectories and put the different chip types in their own directory. > > Best, > > Jim > > > > Kathy Duncan wrote: > > Hi All, > > I've got an Affymetrix dataset which I need to process and > finally get the > list of genes and their respective expression levels. Without > having much > prior idea and experience, I tried the ReadAffy() function > within the > directory containing the .CEL files and I came across the error > saying : > > Error in read.affybatch(filenames = l$filenames, phenoData = > l$phenoData, : > > Cel file MN.CEL does not seem to have the correct dimensions > > Then I tried the following command for each individual .CEL > files of that > investigation (as I am not sure how to get such information > about all the > files using a single command): > > whatcdf ("filename", compress = getOption("BioC")$affy$compress.cel) > > I could see that the investigation is referring to different CDF > files. > > Could you please inform me how I should proceed ! > > kathy > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > <mailto:bioconductor at="" stat.math.ethz.ch=""> > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > > -- > James W. MacDonald, MS > Biostatistician > UMCCC cDNA and Affymetrix Core > University of Michigan > 1500 E Medical Center Drive > 7410 CCGC > Ann Arbor MI 48109 > 734-647-5623 > > -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
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