get probe level intensity from Affymetrix Exon Array platform
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shirley zhang ★ 1.0k
@shirley-zhang-2038
Last seen 10.2 years ago
Dear Bioconductor, I usually obtain exon-level/gene-level signal using Plier/RMA implemented in Affymetrix's Expression Console, however there is no way to get probe level intensity from it. Does anybody know how to get probe level intensity from Affymetrix Exon Array platform? Thanks, Shirley
probe probe • 904 views
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@james-w-macdonald-5106
Last seen 11 hours ago
United States
Hi Shirley, You can get the probe-level data using the exonmap package. Best, Jim shirley zhang wrote: > Dear Bioconductor, > > I usually obtain exon-level/gene-level signal using Plier/RMA > implemented in Affymetrix's Expression Console, however there is no > way to get probe level intensity from it. Does anybody know how to get > probe level intensity from Affymetrix Exon Array platform? > > Thanks, > Shirley > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
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Hi Jim, Thanks for your help. Can you also tell me how to get all of the background probe and positive/negative control probe data using the exonmap package? Shirley On Mon, Mar 17, 2008 at 10:14 AM, James W. MacDonald <jmacdon at="" med.umich.edu=""> wrote: > Hi Shirley, > > You can get the probe-level data using the exonmap package. > > Best, > > Jim > > > > > shirley zhang wrote: > > Dear Bioconductor, > > > > I usually obtain exon-level/gene-level signal using Plier/RMA > > implemented in Affymetrix's Expression Console, however there is no > > way to get probe level intensity from it. Does anybody know how to get > > probe level intensity from Affymetrix Exon Array platform? > > > > Thanks, > > Shirley > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > -- > James W. MacDonald, M.S. > Biostatistician > Affymetrix and cDNA Microarray Core > University of Michigan Cancer Center > 1500 E. Medical Center Drive > 7410 CCGC > Ann Arbor MI 48109 > 734-647-5623 >
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I don't think you can. The exonmap package uses modified cdfs that have all the background and control probesets removed. Best, Jim shirley zhang wrote: > Hi Jim, > > Thanks for your help. Can you also tell me how to get all of the > background probe and positive/negative control probe data using the > exonmap package? > > Shirley > > On Mon, Mar 17, 2008 at 10:14 AM, James W. MacDonald > <jmacdon at="" med.umich.edu=""> wrote: >> Hi Shirley, >> >> You can get the probe-level data using the exonmap package. >> >> Best, >> >> Jim >> >> >> >> >> shirley zhang wrote: >>> Dear Bioconductor, >>> >>> I usually obtain exon-level/gene-level signal using Plier/RMA >>> implemented in Affymetrix's Expression Console, however there is no >>> way to get probe level intensity from it. Does anybody know how to get >>> probe level intensity from Affymetrix Exon Array platform? >>> >>> Thanks, >>> Shirley >>> >>> _______________________________________________ >>> Bioconductor mailing list >>> Bioconductor at stat.math.ethz.ch >>> https://stat.ethz.ch/mailman/listinfo/bioconductor >>> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >> -- >> James W. MacDonald, M.S. >> Biostatistician >> Affymetrix and cDNA Microarray Core >> University of Michigan Cancer Center >> 1500 E. Medical Center Drive >> 7410 CCGC >> Ann Arbor MI 48109 >> 734-647-5623 >> -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
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