How to remove probes from Illumina data?
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@johnstone-alice-2290
Last seen 10.2 years ago
Hello, I have bead-level data from an Illumina expression experiment. I have background corrected and summarised the data, but I need to take the control probes information out of the ExpressionSetIllumina object, or remove them from the beadlevel list. I have tried a few things but they stop me from using the object any further. Could someone please suggest a correct way I could go about this? Thank you. Alice Johnstone PhD Student Institute of Environmental Science and Research Ltd Kenepuru Science Centre 34 Kenepuru Drive PO Box 50-348 Porirua New Zealand Tel: + 64 4 914-0717 Fax: + 64 4 914-0770 P Think before you print This e-mail transmission and any attachments that accompany it may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law and is intended solely for the use of the individual(s) to whom it was intended to be addressed. If you have received this e-mail by mistake, or you are not the intended recipient, any disclosure, dissemination, distribution, copying or other use or retention of this communication or its substance is prohibited. If you have received this communication in error, please immediately reply to the author via e-mail that you received this message by mistake and also permanently delete the original and all copies of this e-mail and any attachments from your computer. Thank you.
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Matt Ritchie ▴ 460
@matt-ritchie-2048
Last seen 10.2 years ago
Hi Alice, You could try setting the 'probes' argument in createBeadSummaryData() - if you set it to a vector containing IDs for the non-control bead types, you should end up with summarised data for these beads only. If this doesn't work for you, or if you tried something else in beadarray that you thought should work, and it failed, please send us an example of the code you ran so that we may fix whatever is wrong. Best wishes, Matt > Hello, > > I have bead-level data from an Illumina expression experiment. I have > background corrected and summarised the data, but I need to take the > control probes information out of the ExpressionSetIllumina object, or > remove them from the beadlevel list. I have tried a few things but they > stop me from using the object any further. Could someone please suggest > a correct way I could go about this? > > Thank you. > > Alice Johnstone
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