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Johnstone, Alice
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410
@johnstone-alice-2290
Last seen 10.2 years ago
Hi there,
My first question is what methods/packages are available for quality
checking of Illumina expression arrays?
This is because I have just received data back from an Illumina Rat
tissue expression experiment and while looking for info on beadlevel
analysis I came across this post:
http://thread.gmane.org/gmane.science.biology.informatics.conductor/15
72
0/focus=15744
In which Matt Richie states that the RegGrn values should be
approximately 1.. for the array data I have back only 2 arrays per
chip
have RegGrn values of 1 the rest sit at ~0.08! Can anyone provide me
with some more information on what the metrics should look like?
I was expecting one of my 3 chips to be of poorer quality but not
all....
Thank you very much!
Alice Johnstone
PhD Student
Institute of Environmental Science and Research Ltd
Kenepuru Science Centre
34 Kenepuru Drive
PO Box 50-348
Porirua
New Zealand
Tel: + 64 4 914-0717
Fax: + 64 4 914-0770
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