RMA bgcorrection question
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@siobhan-a-braybrook-2637
Last seen 10.3 years ago
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Ben Bolstad ★ 1.2k
@ben-bolstad-1494
Last seen 7.3 years ago
No you don't look like an idiot and you are not doing anything wrong. What you are seeing is quite common from the standard implementation. A previous thread discussing this issue https://stat.ethz.ch/pipermail/bioconductor/2006-June/013276.html Best, Ben On Tue, 2008-02-05 at 19:06 -0800, Siobhan A. Braybrook wrote: > Dear all, > > At the risk of looking like an idiot: > is rma background correction supposed to make log-intensity histograms bimodal? > I noticed this in my own data, then checked it using the Dilution set too > (see below). > This seems weird to me........am I doing something wrong? > > data(Dilution) > dil.rma<- bg.correct(Dilution, method="rma") > hist(Dilution[,1:2]) > hist(dil.rma[,1:2]) > > > Thanks! > Siobhan > > S. A. Braybrook > Graduate Student, Harada Lab > Section of Plant Biology > University of California, Davis > Davis, CA 95616 > Ph 530.752.6980 > > The time is always right, to do what is right. > - Martin Luther King, Jr. > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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