On Mon, 4 Aug 2003 bioconductor-request@stat.math.ethz.ch wrote: > Send Bioconductor mailing list submissions to > bioconductor@stat.math.ethz.ch > > To subscribe or unsubscribe via the World Wide Web, visit > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > or, via email, send a message with subject or body 'help' to > bioconductor-request@stat.math.ethz.ch > > You can reach the person managing the list at > bioconductor-owner@stat.math.ethz.ch > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Bioconductor digest..." > > > Today's Topics: > > 1. help on Bioconductor installation (Man, Michael) > 2. gcrma (Crispin Miller) > 3. RNA degradation (again) (Chris Paulse) > 4. how to convert expression set data into matrix data ? > (Anne Sauve) > > > ---------------------------------------------------------------------- > > Message: 3 > Date: Fri, 01 Aug 2003 07:56:47 -0700 > From: "Chris Paulse" <chrispaulse@hotmail.com> > Subject: [BioC] RNA degradation (again) > To: bioconductor@stat.math.ethz.ch > Message-ID: <bay8-f67zatqrqygmji00020162@hotmail.com> > Content-Type: text/plain; format=flowed > > Would it be reasonable to attempt to correct for (normalize) the effects of > RNA degradation? > > Thanks, > Chris Paulse > The p-values in the degradation data table are from a t-test of zero slope. No idea how to interpret them, in the future they will not be included. Better would be to let data from many experiments provide a distribution of slope values and empirical p-values. Correcting for the effects seems to be far away, if possible at all. Leslie Cope