Hi, Please can someone explain what is the difference between Z-factor and Z'-factor as reported in cellHTS2? Many Thanks, Steve

Hi Steve, The difference is that the Z-factor assesses the screening assay quality using the distribution of the control values (either positive or negative controls, depending on the type of the assay) and the samples, while the Z'-factor is based exclusively on controls data using both positive and negative controls. You can find more details in the reference below: JH Zhang, TD Chung, and KR Oldenburg. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen, 4(2):67?73, 1999. Cheers, Ligia > Hi, > > Please can someone explain what is the difference between Z-factor and > Z'-factor as reported in cellHTS2? > > Many Thanks, > > Steve > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor >