Hi People, I am Marcelo and I live in Brazil. Its possible to analyse my datas in bioconductor R package? I have a MacroArray cDNA whith 3840 genes. My experiments: line suscetible control line suscetible treated line resistant control line resistant treated I search in the site of the project, but I was not capable to find the document that I need. Thanks very much Marcelo ---

Dear Marcelo, Have a look at the limma package at http://bioinf.wehi.edu.au/limma/ (and on the Bioconductor site) and the marray packages (on the Bioconductor site) To access documentation, go to www.bioconductor.org and click on "Vignettes" (for the marray pakcages) or see http://bioinf.wehi.edu.au/limma/library/limma/doc/usersguide.html for the limma package. Sandrine Dudoit has some nice PDF slides giving an introduction to Bioconductor and the marray packages, at : http://stat-www.berkeley.edu/~sandrine/Docs/Talks/UCBGenomics03.pdf Also, you can probably find similar introductions on Robert Gentleman's webpage, Wolfgang Huber's, the Bioconductor website etc. Try clicking on "Short Courses" from the bioconductor site www.bioconductor.org ... I have no experience with "Macroarrays" as distinct from "microarrays", but maybe spot-quality weighting is an issue you might want to look it, e.g. see the read.maimages, wtarea, wtflags and wtIgnore.Filter functions in the limma package. I have no experience with "Macroarrays" as distinct from "microarrays", but maybe spot-quality weighting is an issue you might want to look it, e.g. see the read.maimages, wtarea, wtflags and wtIgnore.Filter functions in the limma package. Regards, James > Hi People, > > I am Marcelo and I live in Brazil. > > Its possible to analyse my datas in bioconductor R package? > > I have a MacroArray cDNA whith 3840 genes. > > My experiments: > > line suscetible control > line suscetible treated > line resistant control