Problem with ArrayQuality Package version 1.12
6
0
Entering edit mode
Peter White ▴ 80
@peter-white-2263
Last seen 10.3 years ago
An embedded and charset-unspecified text was scrubbed... Name: not available Url: https://stat.ethz.ch/pipermail/bioconductor/attachments/20070706/ 9ad9dcd7/attachment.pl
• 1.7k views
ADD COMMENT
0
Entering edit mode
Paquet, Agnes ▴ 500
@paquet-agnes-807
Last seen 10.3 years ago
Hi Peter, I just tried to run the function meeboQuality, which calls gpFlagWt, with the same R and BioC setting as you and it worked fine. Could you please email me the R code you are trying to run so that I can reproduce the error? Thank you very much, Agnes ________________________________ From: bioconductor-bounces@stat.math.ethz.ch on behalf of Peter White Sent: Fri 7/6/2007 8:57 AM To: bioconductor at stat.math.ethz.ch Subject: [BioC] Problem with ArrayQuality Package version 1.12 I am running R 2.51 and Bioconductor 2.0. When I attempt to use the arrayQuality package I get the message: Error in do.call("gpFlagWt", list(mraw at maW)) : could not find function "gpFlagWt" I installed a previous version of the package, 1.10, and it works fine as the missing function is included with that download. Could you please update the package or give me a solution to the problem. Thanks, Peter Peter White, Ph.D. Functional Genomics Core Department of Genetics University of Pennsylvania Philadelphia, PA 19104-6145 [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor at stat.math.ethz.ch https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
ADD COMMENT
0
Entering edit mode
Dear Agnes, I was using your maQualityPlots code in my own function (see part of it below). The problem seems to be that in the newer version if I load the arrayQuality library I get an object not found error if I type gpFlagWt or setCtlCol. When I install the old version and type them in it returns the function? Could it be a difference in how it was compiled in R 2.5.1? I can run the maQualityPlots(mrawObj) function just fine with the new version, just not as laid out below. Thanks, Peter fgcQualityPlots <- function (mrawObj, mnormObj = NULL, postProcessed = FALSE, headerInfo = "", save = TRUE, dev = "png", col = NULL, badspotfunction = NULL, controlId = c("ID", "Name"), DEBUG = FALSE, ...) { require(arrayQuality, quietly=TRUE, warn.conflicts=TRUE, keep.source=getOption("keep.source.pkgs")); require(marray, quietly=TRUE, warn.conflicts=TRUE, keep.source=getOption("keep.source.pkgs")); if (!exists("workingDir")) {workingDir = winDialogString("Please enter the working directory:", "D:\\Array_Experiments")} if (!exists("prefixName")) {workingDir = winDialogString("Please enter a Prefix Name for the experiment:", "Experiment")} if (!exists("analysisLog")) analysisLog = FALSE; require(hexbin) if (DEBUG) print("function starting") controlId <- controlId[1] if (setequal(class(mrawObj), "RGList")) { #If your data is a RGList this function will convert it to an marray object mrawTmp <- as(mrawObj, "marrayRaw") mrawObj <- mrawTmp rm(mrawTmp) } if (DEBUG) print(dim(mrawObj)) for (i in 1:dim(mrawObj)[2]) { mraw <- mrawObj[, i] if(!is.null(mnormObj) & postProcessed==TRUE) { mnormObjTmp <- mnormObj[, i] } opt <- list(...) #Function to re-evaluate weights based upon flags if (DEBUG) print("Re-evaluate Weight") if (missing(badspotfunction) || is.null(badspotfunction)) { tmp <- do.call("gpFlagWt", list(mraw at maW)) mraw at maW <- tmp } else if (!is.null(badspotfunction)) mraw at maW <- do.call(badspotfunction, list(mraw at maW)) ifelse(missing(col), colcode <- setCtlCol(mraw), colcode <- col) if (DEBUG) cat("check Control color code", colcode, "\n") -----Original Message----- From: Paquet, Agnes [mailto:apaquet@medsfgh.ucsf.edu] Sent: Friday, July 06, 2007 2:05 PM To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 Hi Peter, I just tried to run the function meeboQuality, which calls gpFlagWt, with the same R and BioC setting as you and it worked fine. Could you please email me the R code you are trying to run so that I can reproduce the error? Thank you very much, Agnes ________________________________ From: bioconductor-bounces@stat.math.ethz.ch on behalf of Peter White Sent: Fri 7/6/2007 8:57 AM To: bioconductor at stat.math.ethz.ch Subject: [BioC] Problem with ArrayQuality Package version 1.12 I am running R 2.51 and Bioconductor 2.0. When I attempt to use the arrayQuality package I get the message: Error in do.call("gpFlagWt", list(mraw at maW)) : could not find function "gpFlagWt" I installed a previous version of the package, 1.10, and it works fine as the missing function is included with that download. Could you please update the package or give me a solution to the problem. Thanks, Peter Peter White, Ph.D. Functional Genomics Core Department of Genetics University of Pennsylvania Philadelphia, PA 19104-6145 [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor at stat.math.ethz.ch https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
ADD REPLY
0
Entering edit mode
Paquet, Agnes ▴ 500
@paquet-agnes-807
Last seen 10.3 years ago
Hi Peter, I just remembered now that we have added a namespace file for arrayQuality in the new release, and the function you are trying to use is not exported anymore. I need to do some check to see if exporting these function would conflit with anything else before I can change the file, so the easiest fix for now would be to add your own flagging and coloring functions in your code. Regards, Agnes ________________________________ From: Peter White [mailto:pwhite@mail.med.upenn.edu] Sent: Fri 7/6/2007 11:56 AM To: Paquet, Agnes; bioconductor at stat.math.ethz.ch Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 Dear Agnes, I was using your maQualityPlots code in my own function (see part of it below). The problem seems to be that in the newer version if I load the arrayQuality library I get an object not found error if I type gpFlagWt or setCtlCol. When I install the old version and type them in it returns the function? Could it be a difference in how it was compiled in R 2.5.1? I can run the maQualityPlots(mrawObj) function just fine with the new version, just not as laid out below. Thanks, Peter fgcQualityPlots <- function (mrawObj, mnormObj = NULL, postProcessed = FALSE, headerInfo = "", save = TRUE, dev = "png", col = NULL, badspotfunction = NULL, controlId = c("ID", "Name"), DEBUG = FALSE, ...) { require(arrayQuality, quietly=TRUE, warn.conflicts=TRUE, keep.source=getOption("keep.source.pkgs")); require(marray, quietly=TRUE, warn.conflicts=TRUE, keep.source=getOption("keep.source.pkgs")); if (!exists("workingDir")) {workingDir = winDialogString("Please enter the working directory:", "D:\\Array_Experiments")} if (!exists("prefixName")) {workingDir = winDialogString("Please enter a Prefix Name for the experiment:", "Experiment")} if (!exists("analysisLog")) analysisLog = FALSE; require(hexbin) if (DEBUG) print("function starting") controlId <- controlId[1] if (setequal(class(mrawObj), "RGList")) { #If your data is a RGList this function will convert it to an marray object mrawTmp <- as(mrawObj, "marrayRaw") mrawObj <- mrawTmp rm(mrawTmp) } if (DEBUG) print(dim(mrawObj)) for (i in 1:dim(mrawObj)[2]) { mraw <- mrawObj[, i] if(!is.null(mnormObj) & postProcessed==TRUE) { mnormObjTmp <- mnormObj[, i] } opt <- list(...) #Function to re-evaluate weights based upon flags if (DEBUG) print("Re-evaluate Weight") if (missing(badspotfunction) || is.null(badspotfunction)) { tmp <- do.call("gpFlagWt", list(mraw at maW)) mraw at maW <- tmp } else if (!is.null(badspotfunction)) mraw at maW <- do.call(badspotfunction, list(mraw at maW)) ifelse(missing(col), colcode <- setCtlCol(mraw), colcode <- col) if (DEBUG) cat("check Control color code", colcode, "\n") -----Original Message----- From: Paquet, Agnes [mailto:apaquet@medsfgh.ucsf.edu] Sent: Friday, July 06, 2007 2:05 PM To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 Hi Peter, I just tried to run the function meeboQuality, which calls gpFlagWt, with the same R and BioC setting as you and it worked fine. Could you please email me the R code you are trying to run so that I can reproduce the error? Thank you very much, Agnes ________________________________ From: bioconductor-bounces@stat.math.ethz.ch on behalf of Peter White Sent: Fri 7/6/2007 8:57 AM To: bioconductor at stat.math.ethz.ch Subject: [BioC] Problem with ArrayQuality Package version 1.12 I am running R 2.51 and Bioconductor 2.0. When I attempt to use the arrayQuality package I get the message: Error in do.call("gpFlagWt", list(mraw at maW)) : could not find function "gpFlagWt" I installed a previous version of the package, 1.10, and it works fine as the missing function is included with that download. Could you please update the package or give me a solution to the problem. Thanks, Peter Peter White, Ph.D. Functional Genomics Core Department of Genetics University of Pennsylvania Philadelphia, PA 19104-6145 [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor at stat.math.ethz.ch https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
ADD COMMENT
0
Entering edit mode
Hi Agnes, That solution worked - I actually had to add the following functions to my code from version 1.10: gpFlagWt, setCtlCol, qpHexbin, qpImage, and qpS2N. Is there anyway to call these functions from the namespace file in the current version? Also, I noticed that arrayQuality is no longer installed with the getBioC() function and it cannot be installed from the "Packages....Install Packages". Is it being removed from the bioconductor package? I hope not as it is very useful and we use it for all our hybs - thanks! Peter -----Original Message----- From: Paquet, Agnes [mailto:apaquet@medsfgh.ucsf.edu] Sent: Friday, July 06, 2007 3:27 PM To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 Hi Peter, I just remembered now that we have added a namespace file for arrayQuality in the new release, and the function you are trying to use is not exported anymore. I need to do some check to see if exporting these function would conflit with anything else before I can change the file, so the easiest fix for now would be to add your own flagging and coloring functions in your code. Regards, Agnes ________________________________ From: Peter White [mailto:pwhite@mail.med.upenn.edu] Sent: Fri 7/6/2007 11:56 AM To: Paquet, Agnes; bioconductor at stat.math.ethz.ch Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 Dear Agnes, I was using your maQualityPlots code in my own function (see part of it below). The problem seems to be that in the newer version if I load the arrayQuality library I get an object not found error if I type gpFlagWt or setCtlCol. When I install the old version and type them in it returns the function? Could it be a difference in how it was compiled in R 2.5.1? I can run the maQualityPlots(mrawObj) function just fine with the new version, just not as laid out below. Thanks, Peter fgcQualityPlots <- function (mrawObj, mnormObj = NULL, postProcessed = FALSE, headerInfo = "", save = TRUE, dev = "png", col = NULL, badspotfunction = NULL, controlId = c("ID", "Name"), DEBUG = FALSE, ...) { require(arrayQuality, quietly=TRUE, warn.conflicts=TRUE, keep.source=getOption("keep.source.pkgs")); require(marray, quietly=TRUE, warn.conflicts=TRUE, keep.source=getOption("keep.source.pkgs")); if (!exists("workingDir")) {workingDir = winDialogString("Please enter the working directory:", "D:\\Array_Experiments")} if (!exists("prefixName")) {workingDir = winDialogString("Please enter a Prefix Name for the experiment:", "Experiment")} if (!exists("analysisLog")) analysisLog = FALSE; require(hexbin) if (DEBUG) print("function starting") controlId <- controlId[1] if (setequal(class(mrawObj), "RGList")) { #If your data is a RGList this function will convert it to an marray object mrawTmp <- as(mrawObj, "marrayRaw") mrawObj <- mrawTmp rm(mrawTmp) } if (DEBUG) print(dim(mrawObj)) for (i in 1:dim(mrawObj)[2]) { mraw <- mrawObj[, i] if(!is.null(mnormObj) & postProcessed==TRUE) { mnormObjTmp <- mnormObj[, i] } opt <- list(...) #Function to re-evaluate weights based upon flags if (DEBUG) print("Re-evaluate Weight") if (missing(badspotfunction) || is.null(badspotfunction)) { tmp <- do.call("gpFlagWt", list(mraw at maW)) mraw at maW <- tmp } else if (!is.null(badspotfunction)) mraw at maW <- do.call(badspotfunction, list(mraw at maW)) ifelse(missing(col), colcode <- setCtlCol(mraw), colcode <- col) if (DEBUG) cat("check Control color code", colcode, "\n") -----Original Message----- From: Paquet, Agnes [mailto:apaquet@medsfgh.ucsf.edu] Sent: Friday, July 06, 2007 2:05 PM To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 Hi Peter, I just tried to run the function meeboQuality, which calls gpFlagWt, with the same R and BioC setting as you and it worked fine. Could you please email me the R code you are trying to run so that I can reproduce the error? Thank you very much, Agnes ________________________________ From: bioconductor-bounces@stat.math.ethz.ch on behalf of Peter White Sent: Fri 7/6/2007 8:57 AM To: bioconductor at stat.math.ethz.ch Subject: [BioC] Problem with ArrayQuality Package version 1.12 I am running R 2.51 and Bioconductor 2.0. When I attempt to use the arrayQuality package I get the message: Error in do.call("gpFlagWt", list(mraw at maW)) : could not find function "gpFlagWt" I installed a previous version of the package, 1.10, and it works fine as the missing function is included with that download. Could you please update the package or give me a solution to the problem. Thanks, Peter Peter White, Ph.D. Functional Genomics Core Department of Genetics University of Pennsylvania Philadelphia, PA 19104-6145 [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor at stat.math.ethz.ch https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
ADD REPLY
0
Entering edit mode
@martin-morgan-1513
Last seen 5 months ago
United States
Hi Peter & Agnes -- variables inside namespaces can be accessed with arrayQuality:::gpFlagWt(mraw at maW) or do.call("gpFlagWt", list(mraw at maW), envir=getNamespace("arrayQuality")) It's probably appropriate not to export these variables from the name space, since they are really implementation details that the user can't realy rely on to stay constant. Martin "Paquet, Agnes" <apaquet at="" medsfgh.ucsf.edu=""> writes: > Hi Peter, > > I just remembered now that we have added a namespace file for > arrayQuality in the new release, and the function you are trying to > use is not exported anymore. I need to do some check to see if > exporting these function would conflit with anything else before I can > change the file, so the easiest fix for now would be to add your own > flagging and coloring functions in your code. > > Regards, > > Agnes > > > > ________________________________ > > From: Peter White [mailto:pwhite at mail.med.upenn.edu] Sent: Fri > 7/6/2007 11:56 AM To: Paquet, Agnes; bioconductor at stat.math.ethz.ch > Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 > > > > Dear Agnes, > > I was using your maQualityPlots code in my own function (see part of > it below). The problem seems to be that in the newer version if I load > the arrayQuality library I get an object not found error if I type > gpFlagWt or setCtlCol. When I install the old version and type them in > it returns the function? > > Could it be a difference in how it was compiled in R 2.5.1? I can run > the maQualityPlots(mrawObj) function just fine with the new version, > just not as laid out below. > > Thanks, > > Peter > > > fgcQualityPlots <- function (mrawObj, mnormObj = NULL, postProcessed = > FALSE, headerInfo = "", save = TRUE, dev = "png", > col = NULL, badspotfunction = NULL, controlId = c("ID", "Name"), > DEBUG = FALSE, ...) { require(arrayQuality, quietly=TRUE, > warn.conflicts=TRUE, keep.source=getOption("keep.source.pkgs")); > require(marray, quietly=TRUE, warn.conflicts=TRUE, > keep.source=getOption("keep.source.pkgs")); if (!exists("workingDir")) > {workingDir = winDialogString("Please enter the working directory:", > "D:\\Array_Experiments")} if (!exists("prefixName")) {workingDir = > winDialogString("Please enter a Prefix Name for the experiment:", > "Experiment")} if (!exists("analysisLog")) analysisLog = FALSE; > > require(hexbin) > if (DEBUG) > print("function starting") > controlId <- controlId[1] > if (setequal(class(mrawObj), "RGList")) { #If your data is a > RGList this function will convert it to an marray object > mrawTmp <- as(mrawObj, "marrayRaw") > mrawObj <- mrawTmp > rm(mrawTmp) > } > if (DEBUG) > print(dim(mrawObj)) > for (i in 1:dim(mrawObj)[2]) { > mraw <- mrawObj[, i] > if(!is.null(mnormObj) & postProcessed==TRUE) { > mnormObjTmp <- mnormObj[, i] > } > opt <- list(...) > > #Function to re-evaluate weights based upon flags > > if (DEBUG) > print("Re-evaluate Weight") > if (missing(badspotfunction) || is.null(badspotfunction)) { > tmp <- do.call("gpFlagWt", list(mraw at maW)) > mraw at maW <- tmp > } > else if (!is.null(badspotfunction)) > mraw at maW <- do.call(badspotfunction, list(mraw at maW)) > > ifelse(missing(col), colcode <- setCtlCol(mraw), colcode <- > col) > if (DEBUG) > cat("check Control color code", colcode, "\n") > > -----Original Message----- From: Paquet, Agnes > [mailto:apaquet at medsfgh.ucsf.edu] Sent: Friday, July 06, 2007 2:05 PM > To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch Subject: > RE: [BioC] Problem with ArrayQuality Package version 1.12 > > Hi Peter, > > I just tried to run the function meeboQuality, which calls gpFlagWt, with > the same R and BioC setting as you and it worked fine. Could you please > email me the R code you are trying to run so that I can reproduce the error? > > > Thank you very much, > > Agnes > ________________________________ > > From: bioconductor-bounces at stat.math.ethz.ch on behalf of Peter White > Sent: Fri 7/6/2007 8:57 AM > To: bioconductor at stat.math.ethz.ch > Subject: [BioC] Problem with ArrayQuality Package version 1.12 > > > > I am running R 2.51 and Bioconductor 2.0. When I attempt to use the > arrayQuality package I get the message: > > > > Error in do.call("gpFlagWt", list(mraw at maW)) : > > could not find function "gpFlagWt" > > > > I installed a previous version of the package, 1.10, and it works fine as > the missing function is included with that download. Could you please update > the package or give me a solution to the problem. > > > > Thanks, > > > > Peter > > > > Peter White, Ph.D. > Functional Genomics Core > Department of Genetics > University of Pennsylvania > Philadelphia, PA 19104-6145 > > > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Martin Morgan Bioconductor / Computational Biology http://bioconductor.org
ADD COMMENT
0
Entering edit mode
@martin-morgan-1513
Last seen 5 months ago
United States
Peter -- something else is up with your system, or did you mean something else? > getBioC('arrayQuality') Running biocinstall version 2.0.8 with R version 2.5.0 Your version of R requires version 2.0 of Bioconductor. trying URL 'http://bioconductor.org/packages/2.0/bioc/src/contrib/arra yQuality_1.12.0.tar.gz' Content type 'application/x-gzip' length 13832851 bytes opened URL ================================================== downloaded 13508Kb * Installing *source* package 'arrayQuality' ... ... Martin "Peter White" <pwhite at="" mail.med.upenn.edu=""> writes: > Hi Agnes, > > That solution worked - I actually had to add the following functions to my > code from version 1.10: gpFlagWt, setCtlCol, qpHexbin, qpImage, and qpS2N. > Is there anyway to call these functions from the namespace file in the > current version? > > Also, I noticed that arrayQuality is no longer installed with the getBioC() > function and it cannot be installed from the "Packages....Install Packages". > Is it being removed from the bioconductor package? I hope not as it is very > useful and we use it for all our hybs - thanks! > > Peter > > -----Original Message----- > From: Paquet, Agnes [mailto:apaquet at medsfgh.ucsf.edu] > Sent: Friday, July 06, 2007 3:27 PM > To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch > Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 > > Hi Peter, > > I just remembered now that we have added a namespace file for arrayQuality > in the new release, and the function you are trying to use is not exported > anymore. I need to do some check to see if exporting these function would > conflit with anything else before I can change the file, so the easiest fix > for now would be to add your own flagging and coloring functions in your > code. > > Regards, > > Agnes > > > > ________________________________ > > From: Peter White [mailto:pwhite at mail.med.upenn.edu] > Sent: Fri 7/6/2007 11:56 AM > To: Paquet, Agnes; bioconductor at stat.math.ethz.ch > Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 > > > > Dear Agnes, > > I was using your maQualityPlots code in my own function (see part of it > below). The problem seems to be that in the newer version if I load the > arrayQuality library I get an object not found error if I type gpFlagWt or > setCtlCol. When I install the old version and type them in it returns the > function? > > Could it be a difference in how it was compiled in R 2.5.1? I can run the > maQualityPlots(mrawObj) function just fine with the new version, just not as > laid out below. > > Thanks, > > Peter > > > fgcQualityPlots <- > function (mrawObj, mnormObj = NULL, postProcessed = FALSE, headerInfo = "", > save = TRUE, dev = "png", > col = NULL, badspotfunction = NULL, controlId = c("ID", "Name"), > DEBUG = FALSE, ...) > { > require(arrayQuality, quietly=TRUE, warn.conflicts=TRUE, > keep.source=getOption("keep.source.pkgs")); > require(marray, quietly=TRUE, warn.conflicts=TRUE, > keep.source=getOption("keep.source.pkgs")); > if (!exists("workingDir")) {workingDir = winDialogString("Please enter the > working directory:", "D:\\Array_Experiments")} > if (!exists("prefixName")) {workingDir = winDialogString("Please enter a > Prefix Name for the experiment:", "Experiment")} > if (!exists("analysisLog")) analysisLog = FALSE; > > require(hexbin) > if (DEBUG) > print("function starting") > controlId <- controlId[1] > if (setequal(class(mrawObj), "RGList")) { #If your data is a RGList > this function will convert it to an marray object > mrawTmp <- as(mrawObj, "marrayRaw") > mrawObj <- mrawTmp > rm(mrawTmp) > } > if (DEBUG) > print(dim(mrawObj)) > for (i in 1:dim(mrawObj)[2]) { > mraw <- mrawObj[, i] > if(!is.null(mnormObj) & postProcessed==TRUE) { > mnormObjTmp <- mnormObj[, i] > } > opt <- list(...) > > #Function to re-evaluate weights based upon flags > > if (DEBUG) > print("Re-evaluate Weight") > if (missing(badspotfunction) || is.null(badspotfunction)) { > tmp <- do.call("gpFlagWt", list(mraw at maW)) > mraw at maW <- tmp > } > else if (!is.null(badspotfunction)) > mraw at maW <- do.call(badspotfunction, list(mraw at maW)) > > ifelse(missing(col), colcode <- setCtlCol(mraw), colcode <- col) > if (DEBUG) > cat("check Control color code", colcode, "\n") > > -----Original Message----- > From: Paquet, Agnes [mailto:apaquet at medsfgh.ucsf.edu] > Sent: Friday, July 06, 2007 2:05 PM > To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch > Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 > > Hi Peter, > > I just tried to run the function meeboQuality, which calls gpFlagWt, with > the same R and BioC setting as you and it worked fine. Could you please > email me the R code you are trying to run so that I can reproduce the error? > > > Thank you very much, > > Agnes > ________________________________ > > From: bioconductor-bounces at stat.math.ethz.ch on behalf of Peter White > Sent: Fri 7/6/2007 8:57 AM > To: bioconductor at stat.math.ethz.ch > Subject: [BioC] Problem with ArrayQuality Package version 1.12 > > > > I am running R 2.51 and Bioconductor 2.0. When I attempt to use the > arrayQuality package I get the message: > > > > Error in do.call("gpFlagWt", list(mraw at maW)) : > > could not find function "gpFlagWt" > > > > I installed a previous version of the package, 1.10, and it works fine as > the missing function is included with that download. Could you please update > the package or give me a solution to the problem. > > > > Thanks, > > > > Peter > > > > Peter White, Ph.D. > Functional Genomics Core > Department of Genetics > University of Pennsylvania > Philadelphia, PA 19104-6145 > > > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Martin Morgan Bioconductor / Computational Biology http://bioconductor.org
ADD COMMENT
0
Entering edit mode
Martin - I installed arrayQuality as you show below, but it did not install automatically with: source("http://www.bioconductor.org/getBioC.R") getBioC() and I am pretty sure it used to. Also, in the R GUI if you select the BioC repositories, then click Packages...Install Packages... arrayQuality is not listed on my system? Thanks, Peter -----Original Message----- From: Martin Morgan [mailto:mtmorgan@fhcrc.org] Sent: Friday, July 06, 2007 4:54 PM To: pwhite at mail.med.upenn.edu Cc: 'Paquet, Agnes'; bioconductor at stat.math.ethz.ch Subject: Re: [BioC] Problem with ArrayQuality Package version 1.12 Peter -- something else is up with your system, or did you mean something else? > getBioC('arrayQuality') Running biocinstall version 2.0.8 with R version 2.5.0 Your version of R requires version 2.0 of Bioconductor. trying URL 'http://bioconductor.org/packages/2.0/bioc/src/contrib/arrayQuality_1. 12.0.t ar.gz' Content type 'application/x-gzip' length 13832851 bytes opened URL ================================================== downloaded 13508Kb * Installing *source* package 'arrayQuality' ... ... Martin "Peter White" <pwhite at="" mail.med.upenn.edu=""> writes: > Hi Agnes, > > That solution worked - I actually had to add the following functions to my > code from version 1.10: gpFlagWt, setCtlCol, qpHexbin, qpImage, and qpS2N. > Is there anyway to call these functions from the namespace file in the > current version? > > Also, I noticed that arrayQuality is no longer installed with the getBioC() > function and it cannot be installed from the "Packages....Install Packages". > Is it being removed from the bioconductor package? I hope not as it is very > useful and we use it for all our hybs - thanks! > > Peter > > -----Original Message----- > From: Paquet, Agnes [mailto:apaquet at medsfgh.ucsf.edu] > Sent: Friday, July 06, 2007 3:27 PM > To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch > Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 > > Hi Peter, > > I just remembered now that we have added a namespace file for arrayQuality > in the new release, and the function you are trying to use is not exported > anymore. I need to do some check to see if exporting these function would > conflit with anything else before I can change the file, so the easiest fix > for now would be to add your own flagging and coloring functions in your > code. > > Regards, > > Agnes > > > > ________________________________ > > From: Peter White [mailto:pwhite at mail.med.upenn.edu] > Sent: Fri 7/6/2007 11:56 AM > To: Paquet, Agnes; bioconductor at stat.math.ethz.ch > Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 > > > > Dear Agnes, > > I was using your maQualityPlots code in my own function (see part of it > below). The problem seems to be that in the newer version if I load the > arrayQuality library I get an object not found error if I type gpFlagWt or > setCtlCol. When I install the old version and type them in it returns the > function? > > Could it be a difference in how it was compiled in R 2.5.1? I can run the > maQualityPlots(mrawObj) function just fine with the new version, just not as > laid out below. > > Thanks, > > Peter > > > fgcQualityPlots <- > function (mrawObj, mnormObj = NULL, postProcessed = FALSE, headerInfo = "", > save = TRUE, dev = "png", > col = NULL, badspotfunction = NULL, controlId = c("ID", "Name"), > DEBUG = FALSE, ...) > { > require(arrayQuality, quietly=TRUE, warn.conflicts=TRUE, > keep.source=getOption("keep.source.pkgs")); > require(marray, quietly=TRUE, warn.conflicts=TRUE, > keep.source=getOption("keep.source.pkgs")); > if (!exists("workingDir")) {workingDir = winDialogString("Please enter the > working directory:", "D:\\Array_Experiments")} > if (!exists("prefixName")) {workingDir = winDialogString("Please enter a > Prefix Name for the experiment:", "Experiment")} > if (!exists("analysisLog")) analysisLog = FALSE; > > require(hexbin) > if (DEBUG) > print("function starting") > controlId <- controlId[1] > if (setequal(class(mrawObj), "RGList")) { #If your data is a RGList > this function will convert it to an marray object > mrawTmp <- as(mrawObj, "marrayRaw") > mrawObj <- mrawTmp > rm(mrawTmp) > } > if (DEBUG) > print(dim(mrawObj)) > for (i in 1:dim(mrawObj)[2]) { > mraw <- mrawObj[, i] > if(!is.null(mnormObj) & postProcessed==TRUE) { > mnormObjTmp <- mnormObj[, i] > } > opt <- list(...) > > #Function to re-evaluate weights based upon flags > > if (DEBUG) > print("Re-evaluate Weight") > if (missing(badspotfunction) || is.null(badspotfunction)) { > tmp <- do.call("gpFlagWt", list(mraw at maW)) > mraw at maW <- tmp > } > else if (!is.null(badspotfunction)) > mraw at maW <- do.call(badspotfunction, list(mraw at maW)) > > ifelse(missing(col), colcode <- setCtlCol(mraw), colcode <- col) > if (DEBUG) > cat("check Control color code", colcode, "\n") > > -----Original Message----- > From: Paquet, Agnes [mailto:apaquet at medsfgh.ucsf.edu] > Sent: Friday, July 06, 2007 2:05 PM > To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch > Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 > > Hi Peter, > > I just tried to run the function meeboQuality, which calls gpFlagWt, with > the same R and BioC setting as you and it worked fine. Could you please > email me the R code you are trying to run so that I can reproduce the error? > > > Thank you very much, > > Agnes > ________________________________ > > From: bioconductor-bounces at stat.math.ethz.ch on behalf of Peter White > Sent: Fri 7/6/2007 8:57 AM > To: bioconductor at stat.math.ethz.ch > Subject: [BioC] Problem with ArrayQuality Package version 1.12 > > > > I am running R 2.51 and Bioconductor 2.0. When I attempt to use the > arrayQuality package I get the message: > > > > Error in do.call("gpFlagWt", list(mraw at maW)) : > > could not find function "gpFlagWt" > > > > I installed a previous version of the package, 1.10, and it works fine as > the missing function is included with that download. Could you please update > the package or give me a solution to the problem. > > > > Thanks, > > > > Peter > > > > Peter White, Ph.D. > Functional Genomics Core > Department of Genetics > University of Pennsylvania > Philadelphia, PA 19104-6145 > > > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Martin Morgan Bioconductor / Computational Biology http://bioconductor.org
ADD REPLY
0
Entering edit mode
@martin-morgan-1513
Last seen 5 months ago
United States
Hi Peter -- "Peter White" <pwhite at="" mail.med.upenn.edu=""> writes: > Martin - I installed arrayQuality as you show below, but it did not install > automatically with: > > source("http://www.bioconductor.org/getBioC.R") > getBioC() Taking a peak at archived versions, I suspect that arrayQuality has not been in the default package list... > and I am pretty sure it used to. Also, in the R GUI if you select the BioC > repositories, then click Packages...Install Packages... arrayQuality is not > listed on my system? ... and I think this is a problem at our (for your version of R, I bet no Bioconductor packages are showing up!); we'll investigate. Martin > Thanks, > > Peter > > > -----Original Message----- > From: Martin Morgan [mailto:mtmorgan at fhcrc.org] > Sent: Friday, July 06, 2007 4:54 PM > To: pwhite at mail.med.upenn.edu > Cc: 'Paquet, Agnes'; bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] Problem with ArrayQuality Package version 1.12 > > Peter -- something else is up with your system, or did you mean > something else? > >> getBioC('arrayQuality') > Running biocinstall version 2.0.8 with R version 2.5.0 > Your version of R requires version 2.0 of Bioconductor. > trying URL > 'http://bioconductor.org/packages/2.0/bioc/src/contrib/arrayQuality_ 1.12.0.t > ar.gz' > Content type 'application/x-gzip' length 13832851 bytes > opened URL > ================================================== > downloaded 13508Kb > > * Installing *source* package 'arrayQuality' ... > ... > > Martin > > "Peter White" <pwhite at="" mail.med.upenn.edu=""> writes: > >> Hi Agnes, >> >> That solution worked - I actually had to add the following functions to my >> code from version 1.10: gpFlagWt, setCtlCol, qpHexbin, qpImage, and qpS2N. >> Is there anyway to call these functions from the namespace file in the >> current version? >> >> Also, I noticed that arrayQuality is no longer installed with the > getBioC() >> function and it cannot be installed from the "Packages....Install > Packages". >> Is it being removed from the bioconductor package? I hope not as it is > very >> useful and we use it for all our hybs - thanks! >> >> Peter >> >> -----Original Message----- >> From: Paquet, Agnes [mailto:apaquet at medsfgh.ucsf.edu] >> Sent: Friday, July 06, 2007 3:27 PM >> To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch >> Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 >> >> Hi Peter, >> >> I just remembered now that we have added a namespace file for arrayQuality >> in the new release, and the function you are trying to use is not exported >> anymore. I need to do some check to see if exporting these function would >> conflit with anything else before I can change the file, so the easiest > fix >> for now would be to add your own flagging and coloring functions in your >> code. >> >> Regards, >> >> Agnes >> >> >> >> ________________________________ >> >> From: Peter White [mailto:pwhite at mail.med.upenn.edu] >> Sent: Fri 7/6/2007 11:56 AM >> To: Paquet, Agnes; bioconductor at stat.math.ethz.ch >> Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 >> >> >> >> Dear Agnes, >> >> I was using your maQualityPlots code in my own function (see part of it >> below). The problem seems to be that in the newer version if I load the >> arrayQuality library I get an object not found error if I type gpFlagWt or >> setCtlCol. When I install the old version and type them in it returns the >> function? >> >> Could it be a difference in how it was compiled in R 2.5.1? I can run the >> maQualityPlots(mrawObj) function just fine with the new version, just not > as >> laid out below. >> >> Thanks, >> >> Peter >> >> >> fgcQualityPlots <- >> function (mrawObj, mnormObj = NULL, postProcessed = FALSE, headerInfo = > "", >> save = TRUE, dev = "png", >> col = NULL, badspotfunction = NULL, controlId = c("ID", "Name"), >> DEBUG = FALSE, ...) >> { >> require(arrayQuality, quietly=TRUE, warn.conflicts=TRUE, >> keep.source=getOption("keep.source.pkgs")); >> require(marray, quietly=TRUE, warn.conflicts=TRUE, >> keep.source=getOption("keep.source.pkgs")); >> if (!exists("workingDir")) {workingDir = winDialogString("Please enter the >> working directory:", "D:\\Array_Experiments")} >> if (!exists("prefixName")) {workingDir = winDialogString("Please enter a >> Prefix Name for the experiment:", "Experiment")} >> if (!exists("analysisLog")) analysisLog = FALSE; >> >> require(hexbin) >> if (DEBUG) >> print("function starting") >> controlId <- controlId[1] >> if (setequal(class(mrawObj), "RGList")) { #If your data is a RGList >> this function will convert it to an marray object >> mrawTmp <- as(mrawObj, "marrayRaw") >> mrawObj <- mrawTmp >> rm(mrawTmp) >> } >> if (DEBUG) >> print(dim(mrawObj)) >> for (i in 1:dim(mrawObj)[2]) { >> mraw <- mrawObj[, i] >> if(!is.null(mnormObj) & postProcessed==TRUE) { >> mnormObjTmp <- mnormObj[, i] >> } >> opt <- list(...) >> >> #Function to re-evaluate weights based upon flags >> >> if (DEBUG) >> print("Re-evaluate Weight") >> if (missing(badspotfunction) || is.null(badspotfunction)) { >> tmp <- do.call("gpFlagWt", list(mraw at maW)) >> mraw at maW <- tmp >> } >> else if (!is.null(badspotfunction)) >> mraw at maW <- do.call(badspotfunction, list(mraw at maW)) >> >> ifelse(missing(col), colcode <- setCtlCol(mraw), colcode <- col) >> if (DEBUG) >> cat("check Control color code", colcode, "\n") >> >> -----Original Message----- >> From: Paquet, Agnes [mailto:apaquet at medsfgh.ucsf.edu] >> Sent: Friday, July 06, 2007 2:05 PM >> To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch >> Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 >> >> Hi Peter, >> >> I just tried to run the function meeboQuality, which calls gpFlagWt, with >> the same R and BioC setting as you and it worked fine. Could you please >> email me the R code you are trying to run so that I can reproduce the > error? >> >> >> Thank you very much, >> >> Agnes >> ________________________________ >> >> From: bioconductor-bounces at stat.math.ethz.ch on behalf of Peter White >> Sent: Fri 7/6/2007 8:57 AM >> To: bioconductor at stat.math.ethz.ch >> Subject: [BioC] Problem with ArrayQuality Package version 1.12 >> >> >> >> I am running R 2.51 and Bioconductor 2.0. When I attempt to use the >> arrayQuality package I get the message: >> >> >> >> Error in do.call("gpFlagWt", list(mraw at maW)) : >> >> could not find function "gpFlagWt" >> >> >> >> I installed a previous version of the package, 1.10, and it works fine as >> the missing function is included with that download. Could you please > update >> the package or give me a solution to the problem. >> >> >> >> Thanks, >> >> >> >> Peter >> >> >> >> Peter White, Ph.D. >> Functional Genomics Core >> Department of Genetics >> University of Pennsylvania >> Philadelphia, PA 19104-6145 >> >> >> >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > -- > Martin Morgan > Bioconductor / Computational Biology > http://bioconductor.org > -- Martin Morgan Bioconductor / Computational Biology http://bioconductor.org
ADD COMMENT
0
Entering edit mode
Paquet, Agnes ▴ 500
@paquet-agnes-807
Last seen 10.3 years ago
Thank you very much for the examples Martin, Agnes -----Original Message----- From: Martin Morgan [mailto:mtmorgan@fhcrc.org] Sent: Fri 7/6/2007 1:37 PM To: Paquet, Agnes Cc: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch Subject: Re: [BioC] Problem with ArrayQuality Package version 1.12 Hi Peter & Agnes -- variables inside namespaces can be accessed with arrayQuality:::gpFlagWt(mraw at maW) or do.call("gpFlagWt", list(mraw at maW), envir=getNamespace("arrayQuality")) It's probably appropriate not to export these variables from the name space, since they are really implementation details that the user can't realy rely on to stay constant. Martin "Paquet, Agnes" <apaquet at="" medsfgh.ucsf.edu=""> writes: > Hi Peter, > > I just remembered now that we have added a namespace file for > arrayQuality in the new release, and the function you are trying to > use is not exported anymore. I need to do some check to see if > exporting these function would conflit with anything else before I can > change the file, so the easiest fix for now would be to add your own > flagging and coloring functions in your code. > > Regards, > > Agnes > > > > ________________________________ > > From: Peter White [mailto:pwhite at mail.med.upenn.edu] Sent: Fri > 7/6/2007 11:56 AM To: Paquet, Agnes; bioconductor at stat.math.ethz.ch > Subject: RE: [BioC] Problem with ArrayQuality Package version 1.12 > > > > Dear Agnes, > > I was using your maQualityPlots code in my own function (see part of > it below). The problem seems to be that in the newer version if I load > the arrayQuality library I get an object not found error if I type > gpFlagWt or setCtlCol. When I install the old version and type them in > it returns the function? > > Could it be a difference in how it was compiled in R 2.5.1? I can run > the maQualityPlots(mrawObj) function just fine with the new version, > just not as laid out below. > > Thanks, > > Peter > > > fgcQualityPlots <- function (mrawObj, mnormObj = NULL, postProcessed = > FALSE, headerInfo = "", save = TRUE, dev = "png", > col = NULL, badspotfunction = NULL, controlId = c("ID", "Name"), > DEBUG = FALSE, ...) { require(arrayQuality, quietly=TRUE, > warn.conflicts=TRUE, keep.source=getOption("keep.source.pkgs")); > require(marray, quietly=TRUE, warn.conflicts=TRUE, > keep.source=getOption("keep.source.pkgs")); if (!exists("workingDir")) > {workingDir = winDialogString("Please enter the working directory:", > "D:\\Array_Experiments")} if (!exists("prefixName")) {workingDir = > winDialogString("Please enter a Prefix Name for the experiment:", > "Experiment")} if (!exists("analysisLog")) analysisLog = FALSE; > > require(hexbin) > if (DEBUG) > print("function starting") > controlId <- controlId[1] > if (setequal(class(mrawObj), "RGList")) { #If your data is a > RGList this function will convert it to an marray object > mrawTmp <- as(mrawObj, "marrayRaw") > mrawObj <- mrawTmp > rm(mrawTmp) > } > if (DEBUG) > print(dim(mrawObj)) > for (i in 1:dim(mrawObj)[2]) { > mraw <- mrawObj[, i] > if(!is.null(mnormObj) & postProcessed==TRUE) { > mnormObjTmp <- mnormObj[, i] > } > opt <- list(...) > > #Function to re-evaluate weights based upon flags > > if (DEBUG) > print("Re-evaluate Weight") > if (missing(badspotfunction) || is.null(badspotfunction)) { > tmp <- do.call("gpFlagWt", list(mraw at maW)) > mraw at maW <- tmp > } > else if (!is.null(badspotfunction)) > mraw at maW <- do.call(badspotfunction, list(mraw at maW)) > > ifelse(missing(col), colcode <- setCtlCol(mraw), colcode <- > col) > if (DEBUG) > cat("check Control color code", colcode, "\n") > > -----Original Message----- From: Paquet, Agnes > [mailto:apaquet at medsfgh.ucsf.edu] Sent: Friday, July 06, 2007 2:05 PM > To: pwhite at mail.med.upenn.edu; bioconductor at stat.math.ethz.ch Subject: > RE: [BioC] Problem with ArrayQuality Package version 1.12 > > Hi Peter, > > I just tried to run the function meeboQuality, which calls gpFlagWt, with > the same R and BioC setting as you and it worked fine. Could you please > email me the R code you are trying to run so that I can reproduce the error? > > > Thank you very much, > > Agnes > ________________________________ > > From: bioconductor-bounces at stat.math.ethz.ch on behalf of Peter White > Sent: Fri 7/6/2007 8:57 AM > To: bioconductor at stat.math.ethz.ch > Subject: [BioC] Problem with ArrayQuality Package version 1.12 > > > > I am running R 2.51 and Bioconductor 2.0. When I attempt to use the > arrayQuality package I get the message: > > > > Error in do.call("gpFlagWt", list(mraw at maW)) : > > could not find function "gpFlagWt" > > > > I installed a previous version of the package, 1.10, and it works fine as > the missing function is included with that download. Could you please update > the package or give me a solution to the problem. > > > > Thanks, > > > > Peter > > > > Peter White, Ph.D. > Functional Genomics Core > Department of Genetics > University of Pennsylvania > Philadelphia, PA 19104-6145 > > > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Martin Morgan Bioconductor / Computational Biology http://bioconductor.org
ADD COMMENT

Login before adding your answer.

Traffic: 675 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6