Illimina, BeadExplorer : getQC problem
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@duhaime-johanne-162
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@nick-matigian-2225
Last seen 10.1 years ago
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@nick-matigian-2225
Last seen 10.1 years ago
Hi Sorry for the previous empty messages, hope this works now. I have been getting the same error, I have run the same csv file and code using a older version of R,does anybody know how to fix? > DATA<-readBead("Illumina Trial Ref- 6v2_Sample_Probe_Profile_Nonorm.csv", annotation="Trial"); Read Illumina Trial Ref-6v2_Sample_Probe_Profile_Nonorm.csv Error in data.frame(sample = 1:length(sampleNames), row.names = sampleNames) : row names supplied are of the wrong length In addition: Warning message: read.phenoData is deprecated, use read.AnnotatedDataFrame instead Thanks Nick --------------------------------------------------------------------- Hello We are trying to analyse some Illumina chips. We have installed BeadExplorer_1.2.0.zip <http: bioconductor.org="" packages="" 2.0="" bioc="" bin="" windows="" contrib="" 2.5="" bea="" de="" xplorer_1.2.0.zip=""> . The BeadStudio version of our datafiles 1.5. We followed the Vignette. we do the following: data<-readBead("060027_PMT 585_gene_profile.csv") Read 060027_PMT 585_gene_profile.csv Error in data.frame(sample = 1:length(sampleNames), row.names = sampleNames) : row names supplied are of the wrong length In addition: Warning message: read.phenoData is deprecated, use read.AnnotatedDataFrame instead > dir() [1] "060027_PMT 585_gene_profile.csv" "060027_PMT 585_gene_profile.txt" "060027_PMT 585_qcinfo.csv" [4] "060027_PMT 585_qcinfo.txt" "060027_PMT 585_short_gene_profile.txt" "BeadExplorer" [7] "qcinfo_headers.txt" Then we tried with tab delimited > data<-readBead("060027_PMT 585_gene_profile.txt") Read 060027_PMT 585_gene_profile.txt Warning messages: 1: read.phenoData is deprecated, use read.AnnotatedDataFrame instead 2: The phenoData class is deprecated, use AnnotatedDataFrame (with ExpressionSet) instead 3: The exprSet class is deprecated, use ExpressionSet instead 4: The exprSet class is deprecated, use ExpressionSet instead 5: The exprSet class is deprecated, use ExpressionSet instead 6: The exprSet class is deprecated, use ExpressionSet instead 7: The exprSet class is deprecated, use ExpressionSet instead 8: The exprSet class is deprecated, use ExpressionSet instead 9: The exprSet class is deprecated, use ExpressionSet instead 10: The exprSet class is deprecated, use ExpressionSet instead >data Expression Set (exprSet) with 47293 genes 9 samples phenoData object with 1 variables and 9 cases varLabels sample: arbitrary numbering Warning messages: 1: The exprSet class is deprecated, use ExpressionSet instead 2: The exprSet class is deprecated, use ExpressionSet instead 3: The phenoData class is deprecated, use AnnotatedDataFrame (with ExpressionSet) instead but: > qcdata<-getQC(data) Error in `row.names<-.data.frame`(`*tmp*`, value = c("A.sister.avant.confluence", : invalid 'row.names' length First line of qc file: 060027_PMT 585_qcinfo.txt Illumina Inc. BeadStudio version 1.5.0.34 Normalization = none Array Content = Human_WG-6.xml Error Model = none DateTime = 30/5/2006 09:51
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