Hi, I am not sure how GOCount numbers are caluclated (for instance what GO release is used,what databases where considered, etc...) and I can not reproduce the GOCount count numbers using Amigo.... For instance for GO:0016772 I get > GOCount["GO:0016772",] all human mouse rat yeast plant microbe GO:0016772 165560 1819 962 602 335 1824 1362 What does the 'all' entry represent? How does it compare with the following AmiGO output (all databases)? GO:0016772 : transferase activity, transferring phosphorus- containing groups [7573] Thanks for any help Ariel./