Hello all again, I am trying to upload some mgu74av2 CEl files from my C:\Program Files\R\Rwork directory I have upgraded my R version to the latest dev version I have Tcl/Tk 8.4 version installed I have affy version 1.1.2 I have downloaded the mouse cdf and probe files When I run the following the ReadAffy works OK > alldata <- ReadAffy() > alldata AffyBatch object size of arrays=640x640 features (60804 kb) cdf=MG_U74Av2 (12488 affyids) number of samples=19 number of genes=12488 annotation=mgu74av2 notes= But when I try the widget option I still get: > alldata <- ReadAffy(widget=TRUE) Error in structure(.External("dotTcl", ..., PACKAGE = "tcltk"), class = "tclObj") : [tcl] ambiguous option "-col": must be -column, -columnspan, -in, -ipadx, -ipady, -padx, -pady, -row, -rowspan, or -sticky. May this have anything to do with the environment variable set up for Tcl/Tk? I have it as follows: Variable name: TCL_LIBRARY Variable value: c:\tcl\lib\tcl8.4. Thanks again for all your suggestions, Fatima _______ ? ? F?tima N??ez, PhD Centre for Cancer Research (CIC) University of Salamanca-CSIC Campus Unamuno 37007 Salamanca?????????????????????????????????????????????????? Spain Phone: + 34 923 294802 Fax:?????+ 34 923 294743 E-mail: fnunez@usal.es