CAGEr promoter width graph
0
0
Entering edit mode
@leehami7407-24156
Last seen 4.3 years ago

Hello, I am currently using CAGEr to analyze CAGE data from Fantom5. I put human fetal brain and human adult brain data set.

cumulativeCTSSdistribution(adultbrainx1, clusters = "tagClusters")
quantilePositions(adultbrainx1, clusters = "tagClusters", qLow = 0.1, qUp = 0.9)
##to get tag clusters and their interquantile width##
tagClustersGR( adultbrainx1, "fetalbrain_reformat_x.bed"
         , returnInterquantileWidth = TRUE,  qLow = 0.1, qUp = 0.9)
tagClustersGR( adultbrainx1, "adultbrain_reformat_x.bed"
         , returnInterquantileWidth = TRUE,  qLow = 0.1, qUp = 0.9)
plotInterquantileWidth(adultbrainx1, clusters = "tagClusters", tpmThreshold = 3, qLow = 0.1, qUp = 0.9)

After this, I only get one plot for fetalbrainreformatx.bed but not adultbrainreformatx.bed

Can I get a help for this?

Thank you,

CAGEr • 1.4k views
ADD COMMENT
0
Entering edit mode

Hello, is there a way you can share your CAGEr object with me?

ADD REPLY
0
Entering edit mode

sure bur how should I share it with you? do you think the object is the problem here?

ADD REPLY
0
Entering edit mode

I need to be able to reproduce the problem on my computer to work on it. The best for me would be if you could prepare a reproducible example with test data. But if this is difficult, I can have a look at your CAGEr object serialised with saveRDS.

ADD REPLY
0
Entering edit mode

I cant see your email address! Could you give me your email address? or is there any other ways to send you my file?

ADD REPLY
0
Entering edit mode

My email address is charles.plessy at oist dot jp (email addresses from package maintainers are listed on the package's page on the Bioconductor website and in the DESCRIPTION page of the package. If it is not too late, please feel free to send me a rds object.

ADD REPLY

Login before adding your answer.

Traffic: 382 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6