Dear Sirs, I would greatly appreciate if you could help me with my problem. I try to read in Genepix data and have a problem with the function read.maimages, even if I list the columns explicitely like this: RG <-read.maimages(files,source="genepix",columns=list(Gf="F532 Mean",Gb="B532 Median"),annotation=c("Block","Column","Row","Name","ID")) I guess I have different column heading in my image output file as is required in the function and some columns are absent. Could you please tell me how I can avoid this problem. Thank you, Svetlana Bulashevska.