Entering edit mode
Jeff Gentry
★
3.9k
@jeff-gentry-12
Last seen 10.1 years ago
(pardon the top posting here)
Dick ...
I'm forwarding this to the Bioconductor mailing list as I honestly
don't
know the answer to this question, but am sure someone here will. :)
-Jeff
On Fri, 24 May 2002, Dick Beyer wrote:
> I am trying out Spot and the Bioconductor package. I like it very
much. However, I am worried about the processing of the tiff files by
Spot. Do you know if Spot can handle the GEL files that are output by
the Molecular Dynamics scanner? These are special format tiff images
with the following characteristics:
>
> Gel files are special format 16 bit TIFF files. Each color is saved
as a
> separate 16 bit grey scale image. In addition, due to the large
dynamic
> range of the scanner (about 6-7 logs) and the limited dynamic range
of a 16
> bit image (0-65,000), the intensity data is saved as the square root
of the
> intensity divided by a scaling factor. The single color images can
be
> viewed in some imaging software (not all image display software
allows the
> display of 16 bit TIFF images) as grey scale images. However,
unless the
> software was written to understand the MD format (e.g. Was written
for
> scientific applications) it is unlikely that the relative intensity
values
> displayed by the software will be the correct values.
>
> If you have any information about these tiff files and Spot, I would
greatly appreciate your sharing that with me.
>
> Thanks,
> Dick
>
> ********************************************************************
***********
> Richard P. Beyer, Ph.D. University of Washington
> Tel.:(206) 616 7378 Environmental Health, Box 354695
> Fax: (206) 685 4696 4225 Roosevelt Way NE, # 100
> Seattle, WA 98105-6099
> ********************************************************************
***********
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