chromVAR computeDeviations error: Error in names(res) <- nms
0
0
Entering edit mode
@mkarimzadeh-22900
Last seen 4.3 years ago

Hello,

I get the following error when running

dev <- computeDeviations(object = rse, annotations = motif_mm)
Error in names(res) <- nms :
  'names' attribute [386] must be the same length as the vector [302]
In addition: Warning message:
stop worker failed:
  attempt to select less than one element in OneIndex

The 386 comes from the number of motifs, but I am confused where 302 is coming from.

Here is the output of traceback():

8: bplapply(X, FUN, ..., BPREDO = BPREDO, BPPARAM = BPPARAM)
7: bplapply(X, FUN, ..., BPREDO = BPREDO, BPPARAM = BPPARAM)
6: bplapply(peak_indices, compute_deviations_single, counts_mat = counts_mat,
       background_peaks = background_peaks, expectation = expectation)
5: bplapply(peak_indices, compute_deviations_single, counts_mat = counts_mat,
       background_peaks = background_peaks, expectation = expectation)
4: compute_deviations_core(counts(object), peak_indices, background_peaks,
       expectation, colData = colData(object), rowData = colData(annotations))
3: .local(object, annotations, ...)
2: computeDeviations(object = rse, annotations = motif_mm)
1: computeDeviations(object = rse, annotations = motif_mm)

Here is the information about my objects:

> motif_mm
class: RangedSummarizedExperiment
dim: 4446140 386
metadata(0):
assays(1): motifMatches
rownames: NULL
rowData names(2): name bias
colnames(386): MA0025.1_NFIL3 MA0030.1_FOXF2 ... MA0909.1_HOXD13
  MA0914.1_ISL2
colData names(1): name
> rse
class: RangedSummarizedExperiment
dim: 4446140 26263
metadata(0):
assays(1): counts
rownames: NULL
rowData names(2): name bias
colnames(26263): AAACGAATCGCATACA-1 AAACGAATCGTCCCAT-1 ...
  TTTGTGTTCCCAGCAG-1 TTTGTGTTCTCGACCT-1
colData names(2): Cell_Type depth

And here is the session info:

R version 4.0.2 (2020-06-22)
Platform: x86_64-conda_cos6-linux-gnu (64-bit)
Running under: Ubuntu 18.04.4 LTS
other attached packages:
 [1] JASPAR2016_1.16.0                 BSgenome.Hsapiens.UCSC.hg38_1.4.3
 [3] BSgenome_1.56.0                   rtracklayer_1.48.0
 [5] Biostrings_2.56.0                 XVector_0.28.0
 [7] SummarizedExperiment_1.18.2       DelayedArray_0.14.1
 [9] matrixStats_0.56.0                Biobase_2.48.0
[11] motifmatchr_1.10.0                chromVAR_1.10.0
[13] SnapATAC_1.0.0                    rhdf5_2.32.2
[15] Matrix_1.2-18                     Seurat_3.2.0
[17] GenomicRanges_1.40.0              GenomeInfoDb_1.24.2
[19] IRanges_2.22.2                    S4Vectors_0.26.1
[21] BiocGenerics_0.34.0               RColorBrewer_1.1-2
[23] scales_1.1.1                      reshape2_1.4.4
[25] ggplot2_3.3.2

Would appreciate any feedback.

All the best, Mehran

chromVAR computeDeviations • 891 views
ADD COMMENT

Login before adding your answer.

Traffic: 822 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6