makeTxDbFromGFF warning meaning
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ribioinfo ▴ 100
@ribioinfo-9434
Last seen 4.3 years ago

Hello, Could you please explain the meaning of this warning:

Warning message:
In .get_cds_IDX(mcols0$type, mcols0$phase) :
  The "phase" metadata column contains non-NA values for features of type
  stop_codon. This information was ignored.

to me that I got using the makeTxDbFromGFF function? Thank you.

GenomicFeatures • 1.4k views
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@james-w-macdonald-5106
Last seen 14 hours ago
United States

See here for the GFF specification, in particular the part about column 8. By definition having anything in column 8 of a GFF for a stop codon is meaningless.

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