Newbie 'rflowcyt' question; problem loading facscan256.fcs
1
0
Entering edit mode
Seth Falcon ★ 7.4k
@seth-falcon-992
Last seen 10.2 years ago
Nolwenn LeMeur <nlemeur at="" fhcrc.org=""> writes: > Hi Ulrik, > > I was not able to reproduce your error. I wonder if everyone is using the exact same dataset? > Ulrik Stervbo wrote: >>Here's what I've done after starting R: >> >> >>>library(rflowcyt) >>>library(Biobase) >>>fcs.loc <- system.file("fcs", package = "rfcdmin") >>>file.location <- paste(fcs.loc, "facscan256.fcs", sep = "/") >>>FC.FCSRobj <- read.FCS(file.location, UseS3 = TRUE, MY.DEBUG = >>>TRUE,fcs.type = NULL) Here's one way to verify: 1. Install the digest package. From R, do: source("http://bioconductor.org/biocLite.R") biocLite("digest") 2. Obtain the md5 hash of the data file. By comparing this output, we'll know whether everyone has the same file to begin with. library("rflowcyt") library("digest") ## Get the path to the data file: dataFile <- system.file("fcs/facscan256.fcs", package="rfcdmin") > dataFile [1] "/Users/seth/RLIB-2.4/rfcdmin/fcs/facscan256.fcs" ## Compute the md5 hash of the file, you should get the _same_ ## string as I do here. If not, we have different files. > digest(dataFile, file=TRUE) [1] "bfc603037460c0023569159c7360be0c" + seth
• 1.1k views
ADD COMMENT
0
Entering edit mode
@nolwenn-lemeur-1609
Last seen 10.2 years ago
Hi Seth, Hi Ulrik, I have verified the md5 hash of the data file on my system and I get the same signature as Seth: > library("digest") > datafile=system.file("fcs/facscan256.fcs",package="rfcdmin") > digest(datafile,file=TRUE) [1] "bfc603037460c0023569159c7360be0c" Nolwenn *********************************** Nolwenn Le Meur, PhD Fred Hutchinson Cancer Research Center Computational Biology 1100 Fairview Ave. N., M2-B876 P.O. Box 19024 Seattle, WA 98109-1024 On Mon, 29 May 2006, Seth Falcon wrote: > Nolwenn LeMeur <nlemeur at="" fhcrc.org=""> writes: > > Hi Ulrik, > > > > I was not able to reproduce your error. > > I wonder if everyone is using the exact same dataset? > > > Ulrik Stervbo wrote: > >>Here's what I've done after starting R: > >> > >> > >>>library(rflowcyt) > >>>library(Biobase) > >>>fcs.loc <- system.file("fcs", package = "rfcdmin") > >>>file.location <- paste(fcs.loc, "facscan256.fcs", sep = "/") > >>>FC.FCSRobj <- read.FCS(file.location, UseS3 = TRUE, MY.DEBUG = > >>>TRUE,fcs.type = NULL) > > Here's one way to verify: > > 1. Install the digest package. From R, do: > > source("http://bioconductor.org/biocLite.R") > biocLite("digest") > > 2. Obtain the md5 hash of the data file. By comparing this output, > we'll know whether everyone has the same file to begin with. > > library("rflowcyt") > library("digest") > > ## Get the path to the data file: > dataFile <- system.file("fcs/facscan256.fcs", package="rfcdmin") > > dataFile > [1] "/Users/seth/RLIB-2.4/rfcdmin/fcs/facscan256.fcs" > > ## Compute the md5 hash of the file, you should get the _same_ > ## string as I do here. If not, we have different files. > > digest(dataFile, file=TRUE) > [1] "bfc603037460c0023569159c7360be0c" > > + seth > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
ADD COMMENT
0
Entering edit mode
Hi Seth, Hi Nolwenn I get the same signature as well: > library("digest") > dataFile <- system.file("fcs/facscan256.fcs", package="rfcdmin") > dataFile [1] "/usr/lib/R/library/rfcdmin/fcs/facscan256.fcs" > digest(dataFile, file=TRUE) [1] "bfc603037460c0023569159c7360be0c" I have not yet tried to load my own datafiles, mainly because I am not really sure how. Performing the few examples in the prada package works without problems (but then again the readFCS methods are probably not the same) I have been wanting to see if I could reproduce the error on windows. However, that as well as testing the new lib may be a few days, since other things (laboratory work) are demanding my attention. I will get back with the results as soon as they get in. Thanks Ulrik On 5/29/06, Nolwenn LeMeur <nlemeur at="" fhcrc.org=""> wrote: > Hi Seth, Hi Ulrik, > > I have verified the md5 hash of the data file on my system and I get the > same signature as Seth: > > > library("digest") > > datafile=system.file("fcs/facscan256.fcs",package="rfcdmin") > > digest(datafile,file=TRUE) > [1] "bfc603037460c0023569159c7360be0c" > > > > Nolwenn > > *********************************** > Nolwenn Le Meur, PhD > Fred Hutchinson Cancer Research Center > Computational Biology > 1100 Fairview Ave. N., M2-B876 > P.O. Box 19024 > Seattle, WA 98109-1024 > > On Mon, 29 May 2006, Seth Falcon wrote: > > > Nolwenn LeMeur <nlemeur at="" fhcrc.org=""> writes: > > > Hi Ulrik, > > > > > > I was not able to reproduce your error. > > > > I wonder if everyone is using the exact same dataset? > > > > > Ulrik Stervbo wrote: > > >>Here's what I've done after starting R: > > >> > > >> > > >>>library(rflowcyt) > > >>>library(Biobase) > > >>>fcs.loc <- system.file("fcs", package = "rfcdmin") > > >>>file.location <- paste(fcs.loc, "facscan256.fcs", sep = "/") > > >>>FC.FCSRobj <- read.FCS(file.location, UseS3 = TRUE, MY.DEBUG = > > >>>TRUE,fcs.type = NULL) > > > > Here's one way to verify: > > > > 1. Install the digest package. From R, do: > > > > source("http://bioconductor.org/biocLite.R") > > biocLite("digest") > > > > 2. Obtain the md5 hash of the data file. By comparing this output, > > we'll know whether everyone has the same file to begin with. > > > > library("rflowcyt") > > library("digest") > > > > ## Get the path to the data file: > > dataFile <- system.file("fcs/facscan256.fcs", package="rfcdmin") > > > dataFile > > [1] "/Users/seth/RLIB-2.4/rfcdmin/fcs/facscan256.fcs" > > > > ## Compute the md5 hash of the file, you should get the _same_ > > ## string as I do here. If not, we have different files. > > > digest(dataFile, file=TRUE) > > [1] "bfc603037460c0023569159c7360be0c" > > > > + seth > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > -- Ulrik Stervbo Cantianstrasse 23 10437 Berlin Germany Home: +49 30 441 12 81 Handy: +49 178 145 82 31 Blog: http://ulrikstervbo.blogspot.com Mailing-list: http://www.coollist.com/group.cgi?l=ulrik_i_berlin
ADD REPLY

Login before adding your answer.

Traffic: 546 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6